English
Albanian
Arabic
Armenian
Azerbaijani
Belarusian
Bengali
Bosnian
Catalan
Czech
Danish
Deutsch
Dutch
English
Estonian
Finnish
Français
Greek
Haitian Creole
Hebrew
Hindi
Hungarian
Icelandic
Indonesian
Irish
Italian
Japanese
Korean
Latvian
Lithuanian
Macedonian
Mongolian
Norwegian
Persian
Polish
Portuguese
Romanian
Russian
Serbian
Slovak
Slovenian
Spanish
Swahili
Swedish
Turkish
Ukrainian
Vietnamese
Български
中文(简体)
中文(繁體)
Theriogenology 2016-Dec

Antioxidant protection and lipid peroxidation in testes and different parts of epididymis in boars.

Only registered users can translate articles
Log In/Sign up
The link is saved to the clipboard
Ivona Žura Žaja
Marko Samardžija
Silvijo Vince
Anamaria Sluganović
Sara Strelec
Jelena Šuran
Igor DelVechio
Dražen Đuričić
Mario Ostović
Hrvoje Valpotić

Keywords

Abstract

The antioxidative ability of testes and epididymis to protect spermatozoa from detrimental effects of oxidation processes induced by excessive generation of reactive oxygen species has not been previously studied in detail in boar reproductive tissues. The aim of this study was to investigate differences in level of antioxidative protection, intensity of lipid peroxidation, and values of biochemical parameters in testes and different parts of epididymis in sexually mature boars. The study was performed on five Swedish landrace boars from the same litter aged 10 months kept under same ambient conditions. After slaughtering performed at the end of November; tissue samples of testes and the head, body and tail of epididymis were taken. The activity of glutathione peroxidase, glutathione reductase, superoxide dismutase, γ-glutamyltransferase, acid phosphatase, and lactate dehydrogenase, and concentrations of triacylglycerol, phospholipids, cholesterol, free fatty acids were determined in obtained supernatants from homogenized tissues spectrophotometrically; the concentration of malondialdehyde was determined by high-performance liquid chromatography. Significantly higher activities of glutathione peroxidase and glutathione reductase (P < 0.05) were found in testes compared with epididymis. In testes, a significantly higher activity of superoxide dismutase was found than in the head and tail of epididymis (P < 0.05). Malondialdehyde concentration in head of epididymis was significantly higher than in testes, or the body and tail of epididymis (P < 0.05). Simultaneously, malondialdehyde concentration in testes was significantly higher than in body and tail of epididymis (P < 0.05). In tail of epididymis, significantly higher activities of γ-glutamyltransferase, acid phosphatase, and lactate dehydrogenase were recorded than in testes. Significantly lower concentrations of triacylglycerol and free fatty acids were recorded in epididymis tail in comparison to epididymis head (P < 0.05). It could be concluded that high activities of antioxidative enzymes in testes of boars are essential for the appropriate protection of spermatozoa and cells of testes tissue against oxidative damages. The tissues of testes and epididymis head in boars were more susceptible to lipid peroxidation. Results of the present study indicated physiological importance of antioxidative enzymes in reproductive system in boars, and thus may serve for better understanding the mechanisms of male infertility.

Join our facebook page

The most complete medicinal herbs database backed by science

  • Works in 55 languages
  • Herbal cures backed by science
  • Herbs recognition by image
  • Interactive GPS map - tag herbs on location (coming soon)
  • Read scientific publications related to your search
  • Search medicinal herbs by their effects
  • Organize your interests and stay up do date with the news research, clinical trials and patents

Type a symptom or a disease and read about herbs that might help, type a herb and see diseases and symptoms it is used against.
*All information is based on published scientific research

Google Play badgeApp Store badge