Journal of family medicine and primary care 2019-Aug
Association of passive smoking with dental caries and salivary biomarkers among 5-10 years old children of Muradnagar, Ghaziabad.
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Keywords
Abstract
Methods
A case-control study was conducted among 160 children of age group 5-10 years who visited the outdoor patient department of a dental college. Regular smoking households were recognized and children who lived in smoking households were identified as PS subjects. Two categories of children were formed - PS (80 children) and control group (80 children). Parents completed a pretested questionnaire and clinical examination of children was done using dmft index and gingival index. This was followed by collection of stimulated saliva of children which was further subjected to determine salivary buffering capacity and pH. Inoculation on mitis salivarius-bacitracin agar for counting streptococcus colonies and Rogosa SL agar (Difco) for counting lactobacillus colonies was done. Cotinine level was then measured using enzyme-linked immunosorbent assay kit. Student's independent t-test, Mann-Whitney U test, and one-way analysis of variance test were used for analyzing data.