Bioscience, Biotechnology and Biochemistry 2011

Characterization of recombinant β-amylases from Oryza sativa.

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Tomojiro Koide

Yasuo Ohnishi

Sueharu Horinouchi

ARTICLE: 21512221

DOI: 10.1271/bbb.100872

BioSeek: 21512221

Keywords

Abstract

Four putative β-amylase genes found in the Oryza sativa cDNA sequence database (KOME) were expressed in Escherichia coli. Recombinant proteins from two of these genes showed β-amylase activity. Similarly to β-amylases from other plants, the optimum pH of the recombinant rice β-amylases was about 5.5-6.0, but they exhibited inferior heat stability to soybean β-amylase.

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Characterization of recombinant β-amylases from Oryza sativa.

Keywords

beta amylase

amylase

oryza

oryza sativa

Abstract

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