Evaluation of chemical analysis for the determination of solasodine in Solanum laciniatum.

A detailed study of a method for solasodine analysis has been carried out and the suitability of chemical analysis for solasodine determination in plant material evaluated. A number of problems with the analytical isolation of solasodine and its subsequent colorimetric determination have been highlighted: oven drying of plant material greater than 100 degrees leads to solasodine loss; cell disruption of the dry plant material is required if complete and rapid extraction of solasodine is to take place; hydrolysis of plant extract residues in greater than 1 N acid leads to solasodine loss; the colorimetric procedure is more temperamental than past methods have indicated, especially with regard to the specificity of the reaction and the instability of the complex.