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3 Biotech 2017-Jun

Fabrication, optimization, and characterization of noble silver nanoparticles from sugarcane leaf (Saccharum officinarum) extract for antifungal application.

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Manikandan Velu
Jeong-Ho Lee
Woo-Suk Chang
Nanh Lovanh
Yool-Jin Park
Palaniyappan Jayanthi
Velmurugan Palanivel
Byung-Taek Oh

Keywords

Abstract

Metal nanoparticles obtained from green route are gaining significant prominence as a result of their potential applications in nanomedicine and material engineering. Overall metal nanoparticles studied, silver nanoparticles (AgNPs) clutch prominent place in nanoparticles research field. Herein, we have reported the green synthesis of Saccharum officinarum leaf biomass extract-mediated synthesis of AgNPs. Initial nanoparticle production was confirmed by visual observation as color change from light yellow to bright brown color with yellow shade and spectrophotometrically at 450 nm and the various reaction conditions were optimized. The FTIR spectra of the biomass extract and synthesized AgNPs authorized the presence of phyto constituents as capping agent. The High Resolution-Transmission Electron Microscopy (HR-TEM) analyses confirm the morphology and the average particle size of AgNPs as ~28.2 nm. The crystalline nature oxide state and mean particle diameter of AgNPs were confirmed by X-ray diffraction (XRD) analysis, Selected Area Electron Diffraction (SAED) pattern and face-centered cubic (FCC). The obtained AgNPs show moderate to good antifungal activity against Phytophthora capsici, Colletotrichum acutatum and Cladosporium fulvum as 10, 12 and 14 mm zones of inhibition against synthesized AgNPs at 250 μg/well, respectively.

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