[GC-MS analysis of low polar components from Ainsliaea yunnanensis].

OBJECTIVE

To analyze the chemical constituents of low polar components from ethanol extract of Ainsliaea yunnanensis.

METHODS

The air-dried stems and leaves of Ainsliaea yunnanensis were extracted with ethanol using ultrasonic extration. After removal of solvent under reduced pressure, the residue was suspended in H2O, and then partitioned sequentially with petroleum ether, EtOAc and n-BuOH, respectively. Then the petroleum ether fraction was chromatographed on normal phase silica gels eluting with a gradient increasing Me2 CO in petroleum ether. Then the components of low polarity were separated from the petroleum ether-Me2CO(50:1 - 20: 1) fraction and identified by GC-MS, and the relative contents of the components were determined with area percentage method.

RESULTS

50 constituents were identified from the low polar components of Ainsliaea yunnanensis.

CONCLUSIONS

The research provides a theoretical basis for the study of chemical constituents and pharmaceutical activities of Ainsliaea yunnanensis.