[GC/MS-based dynamic analysis of Eucalyptus camaldulensis extract on correlation of chromatography-activity against Oncomelania hupensis].

OBJECTIVE

To determine the contributions of main chemical compositions of extracts of Eucalyptus camaldulensis represented by GC/MS elute peaks to the molluscicidal activities, and explore a shortcut of looking for the effective components from natural products.

METHODS

E. camaldulensis leaves were collected consecutively in 12 months at the same place, extracted with dichloromethane, analyzed by GC/MS, and their LC50(s) of molluscicidal activities were tested according to the method recommended by WHO. The correlation of the main components in 12 extracts and their molluscicidal activities were analyzed by the grey relative correlation analysis model with software GTMS 3.0.

RESULTS

All the dichloromethane extracts of eucalyptus leaves showed excellent molluscicidal activities with the highest LC50 of 0.257mg/L and 0.242mg/L for the samples in June and July and the lowest LC50 of 6.802 mg/L and 5.406 mg/L in December and January respectively. The structures of 16 main chemical components were elucidated by GC/MS and NIST Mass Spectral Library, most of which were monoterpenes and sesquiterpenoids. The gray correlation coefficients with activity were all over 0.5, the first five over 0.9 were 4,4, 8-Trimethyltricyclo [6.3.1.0 (1,5) ]dodecane-2,9-diol, (-)-Spathulenol, a structural isomer of (-)-Spathulenol, Eucalyptol and Ledol.

CONCLUSIONS

The most main ingredients in the dichloromethane extracts of E. camaldulensis leaves show good correlations with the molluscicidal activity, which suggests that the molluscicidal role is synergistically played by the multiple components together.