Molecular biology of circulatory shock: IV. Translation and secretion of HEP G2 cell proteins are independently attenuated during heat shock.
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Abstract
In previous studies we have shown evidence of heat shock gene transcription in pig liver after cardiogenic shock (Buchman et al: Surgery 108:559, 1990). To study the effect of heat shock on synthesis, secretion, and glycosylation of liver cell proteins, a human hepatoblastoma cell line (Hep G2) was used as a model system. Cells were thermally stressed and incorporation of radioactive amino acids or carbohydrates was studied. Heat shock caused a tenfold decrease in the rate of overall protein synthesis; secretion was attenuated to a lesser extent; glycosylation was spared. Heat shock gene expression is associated with a precipitous and sustained decline in the synthesis and secretion of constitutive proteins which may be critical to recovery from circulatory shock.