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Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 2007-Jun

Natural compounds of Palestine flora. Comparison analysis by static headspace and steam distillation GC-MS of semivolatile secondary metabolites from leaves of cultivated Palestinian Majorana syriaca.

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Saleh Abu-Lafi
Imad Odeh
Hasan Dewik
Mohammed Qabajah
Amin Imam
Valery M Dembitsky
Lumir O Hanus

Keywords

Abstract

BACKGROUND

A comparative analysis by using static headspace (HS) and steam distillation (SD) GC-MS of the volatile and the semi-volatile secondary metabolites from leaves of cultivated Majorana syriaca.

METHODS

The essential oils endogenous to cultivated thyme were isolated and identified by HS-GC-MS technology and compared to those from SD-GC-MS.

RESULTS

The HS-GC-MS results showed that the Palestinian cultivated thyme is rich in monoterpene hydrocarbons and phenolic monoterpenes such as alpha-phellandrene, alpha-pinene, beta-myrcene, m-cymene, p-cymene, gamma-terpinene, thymol and carvacrol. In all the samples gamma-terpinene, p-cymene, thymol and carvacrol were the most abundant compounds.

CONCLUSIONS

HS and SD-GC-MS have proved that most of the cultivated thyme samples examined has thymol isomer as the major phenolic constituent.

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