Journal of Separation Science 2007-Nov
One step purification of flavanone glycosides from Poncirus trifoliata by centrifugal partition chromatography.
Only registered users can translate articles
Log In/Sign up
The link is saved to the clipboard
Keywords
Abstract
Flavanone glycosides were successfully separated from the crude extract of Poncirus trifoliata by preparative centrifugal partition chromatography with a two-phase solvent system composed of ethyl acetate-acetonitrile-water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg), and poncirin (71.9 mg) were purified from the 524 mg crude extract in only one step. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by (1)H NMR, (13)C NMR, and ESI-MS.