Phenolic compounds from Artemisia iwayomogi and their effects on osteoblastic MC3T3-E1 cells.

One new (4) and twelve known phenolic compounds (1-3, 5-13) were isolated from a 70% MeOH extract of the aerial parts of Artemisia iwayomogi. The new compound was identified as 7,8-dimethoxy-coumarin-9-O-(6'-O-(E)-coumaroyl)-beta-D-glucopyranoside (4) and named iwayomin. The effects of compounds 1-13 on the function of osteoblastic MC3T3-E1 cells were examined by evaluating cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization in the presence of each compound. Compounds 3, 4, 7, and 9 showed potential in stimulating osteoblastic bone formation and may be useful for the prevention and/or treatment of osteoporosis.