Madhuca longifolia embedded silver nanoparticles attenuates diethylnitrosamine (DEN) -induced renal cancer via regulating oxidative stress

Objective: Madhuca longifolia has been used for the treatment of renal cancer. Therefore, the current study describes the protective effects of biofabricated silver nanoparticles (MLAgNPs) using Madhuca longifolia aqueous leaves extract against diethylnitrosamine (DEN) induced renal cell carcinoma (RCC) in rats.

Methods: Animals were categorized into five groups and treated with doses of silver nanoparticles for 16 weeks. Antineoplastic effect in renal cancer was dose dependent to control the macroscopical variations when compared to DEN induced group. Significant changes were observed in biochemical parameters and dose graded improvement in the level of antioxidants parameters were accountable for its protective nature.

Result: Silver nanoparticles in dose dependent manner was effective to modify the raised levels of pro-inflammatory cytokines and inflammatory mediators during renal cancer. Alteration in renal histopathology were also detected in the silver nanoparticles treated group, which show its safety concern. Biofabricated silver nanoparticles (MLAgNPs) using Madhuca longifolia can convey significant chemo-protective effect against renal cancer by suppressing the IL-6, TNF-α and IL-1β by nuclear factor-kappa B (NF-κB) pathway.

Conclusion: Our outcomes implicates that biofabricated MLAgNPs exhibited a chemoprotective potential in the prevention and intervention of RCC.

Keywords: Anti-neoplastic; Biofabricated silver nanoparticles; Madhuca longifolia; antioxidants; diethylnitrosamine; renal cancer.