Immuno-gold labelling using a monoclonal antibody (PCBC3) with a primary specificity for α-L-arabinofuranosyl residues was used to locate these residues in pollen tubes of Nicotiana alata grown in vivo. The antibody bound to the outer fibrillar layer of the pollen-tube wall: the inner, non-fibrillar
The effect on in vitro pollen tube growth of an isolated style glycoprotein (S(2)-glycoprotein) associated with self-incompatibility in Nicotiana alata was investigated. Tube growth of pollen bearing the S(2)-allele was inhibited, but tube growth of pollen bearing other alleles was not affected.
An indirect method of quantifying in vitro pollen growth has been developed. This is based on the finding that a monoclonal antibody (PCBC3), which has a primary specificity for alpha-l-arabinofuranosyl residues, binds to the surface of in vitro grown pollen tubes of the ornamental tobacco,
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