Five per cent (w/v) extracts of defatted Ambrosia elatior pollen were prepared in bicarbonate-buffered saline (Coca's extract) and Coca's extract with 50% glycerol (Gly-Coca's extract). Sterile extracts were stored in untreated glassware and glassware coated with silicone. Stock extracts were
OBJECTIVE
To clone the pollen allergen genes in Humulus scandens (Lour) Merr (LvCao in Chinese) and short ragweed (Ambrosia artemisiifolia L) for recombinant allergen production and immunotherapy.
METHODS
The allergen genes were selectively amplified in the weed pollen cDNA pool by using a special
Artemisinin, also called qinghaosu, is originally derived from the sweet wormwood plant (Artemisia annua), which is used in traditional Chinese medicine. Artemisinin and its derivatives (artemisinins) have been widely used for many years as anti-malarial agents, with few adverse side effects.
Ragweed (Ambrosia) pollens contain a number of proteins that cause allergic disease in ragweed-sensitive people. The cloning of the AmbtV cDNA is important, since the 4.4-kDa AmbtV, one of the allergens in giant ragweed (Ambrosia trifida) pollen, serves as a simple model system to study the basic
UNASSIGNED
Allergy is an endemic disease and has a considerable impact on the quality of life. This study aimed to measure the effect of active allergic rhinitis on memory functions of physically active and inactive patients with ragweed allergy.
UNASSIGNED
Memory functions were assessed before and
Deep level insect relationships are generally difficult to resolve, especially within taxa of the most diverse and species rich holometabolous orders. In beetles, the major diversity occurs in the Phytophaga, including charismatic groups such as leaf beetles, longhorn beetles and weevils. Bark and
OBJECTIVE
To obtain the entire gene open reading frame (ORF) and to construct the expression vectors for recombinant allergen production.
METHODS
Gene fragments corresponding to the gene specific region and the cDNA ends of pollen allergens of short ragweed (Rg, Ambrosia artemisiifolia L.) were
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