A high-performance liquid chromatographic (HPLC) method for the assay of the hepatic microsomal polysubstrate monooxygenase catalyzed hydroxylation of testosterone is described. The metabolites are extracted from the incubation mixture with dichloromethane and the extract is washed with dilute
Accurate separation and identification of steroids from the postvitellogenic ovarian follicles of Indian climbing perch Anabas testudineus was performed by high-performance liquid chromatography (HPLC) and specific radioimmunoassay (RIA). The steroids from such follicles, incubated in Cortland's
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