cellulose/infarction

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Cellulose acetate electrophoresis of creatine phosphokinase isoenzymes in the diagnosis of myocardial infarction.

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A method for rapid separation and visualization of creatine phosphokinase (EC 2.7.3.2 ATP: creatine phosphotransferase) isoenzymes on cellulose acetate has been developed. The technic uses the Beckman microzone electrophoresis system, and is applicable to other cellulose acetate systems. Incubation

Fiber intake and risk of nonfatal acute myocardial infarction.

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OBJECTIVE To investigate the association between fiber intake and risk of acute myocardial infarction (AMI), also according to type and source of fiber, in a Mediterranean country. METHODS Hospital-based case-control study. METHODS Northern Italy. METHODS A total of 507 cases of first nonfatal AMI

[Changes in DNA repair enzymes in rat ventroposterior nucleus of the thalamus after cerebral cortex infarction].

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OBJECTIVE To investigate the damage within the ventroposterior nucleus (VPN) of the thalamus after focal cortical infarction and its mechanism, and explore the effect of ebselen on the oxidative damage after cerebral cortex infarction in hypertensive rats. METHODS Middle cerebral artery occlusion

Cellulose acetate and electroendosmosis-low agarose electrophoresis: advanced methods for the separation and quantitative determination of serum creatine kinase isoenzyme levels.

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Two methods for the separation and demonstration of creatine kinase isoenzymes are described i.e. electrophoresis on cellulose acetate and on electroendosmosis-low agarose. The fluorescence of NADPH as an indicator for the creatine kinase bands was used in both methods. The methods proved to be

Measurement of creatine kinase Z in human sera using a DEAE-cellulose mini-column method.

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A DEAE-cellulose mini-column method has been developed which allows for the quantitation in human serum of creatine kinase Z, a sub-band of creatine kinase first described by Lim ((1975) Clin. Chem. 21, 975, Abstract 181) and Sax et al. (Sax, S.M., Moore, J.J., Giegel, J.L. and Welsh, M. (1976)

[Separation of glycogen phosphorylase isoenzymes using electrophoresis in acetate cellulose].

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A new electrophoretic procedure is developed for estimation of glycogen phosphorylase isoforms using acetate cellulose films. In the course of incubation of a mixture containing substrate the reaction was directed towards formation of glucose-I-phosphate, concentration of which was estimated in the

[Separation of alpha-amylase isoenzymes using cellulose acetate electrophoresis].

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alpha-Amylase isozymes were separated by electrophoresis in cellulose acetate to detect the isoforms of the chromogenic substrate manufactured by Lachema, Czechoslovakia. In a group of 20 normal subjects aged 25 to 45, alpha-amylase pancreatic isozyme activity prevailed. Patients with acute

Falsely negative laboratory diagnosis for myocardial infarction owing to the concurrent presence of macro creatine kinase and macro lactate dehydrogenase.

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Macro creatine kinase (CK, EC 2.7.3.2) and macro lactate dehydrogenase (LD, EC 1.1.1.27) were both present in the serum of a 70-year-old woman with myocardial infarction. This interfered with the interpretation of the CK and LD isoenzyme analyses. Gel filtration and immunoprecipitation showed that

[Erythrocyte deformability in patients with acute myocardial infarction].

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A change in filterability of erythrocytes through a cellulose filter with pores 7 microns in diameter was examined in 67 patients with acute myocardial infarction. Dynamic evaluation of the filterability was compared with a process of necrotic focus development in time. The filterability was

Corydalis yanhusuo rhizoma extract reduces infarct size and improves heart function during myocardial ischemia/reperfusion by inhibiting apoptosis in rats.

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The aim of the present investigation was to evaluate the effect of an extract from Corydalis yanhusuo W.T., a Chinese herbal medicine, on ischemia/reperfusion (I/R) injury and to determine the mechanism(s) involved. In rats, the left anterior descending (LAD) coronary artery was occluded for 30 min

Profiling the immune responses of human patients treated with recombinant streptokinase after myocardial infarct.

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The SPOT synthesis of peptide arrays on continuous cellulose membranes should be generally applicable in the analysis of sequential antibody binding sites using the enzyme-substrate or other standard detection protocols. The use of total serum is limited by the occurrence of high background levels.

Hemoperfusion in a child who ingested diquat and died from pontine infarction and hemorrhage.

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A 2 1/2 year old boy accidentally ingested the herbicide diquat. Progressive neurologic dysfunction preceded his death 143 hours after poisoning. Brain stem infarction and purpura were noted at post mortem and closely resembled the brain stem pathology in 3 of 7 adults who died after diquat

The effect of a peptide-modified thermo-reversible methylcellulose on wound healing and LV function in a chronic myocardial infarction rodent model.

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Myocardial infarction is the main contributor to heart failure. In this study we examined whether modification of a thermo-reversible cellulose-based polymer with extracellular-matrix derived functional groups could promote wound healing and improve cardiac function in a chronic rodent model of

CPK and CPK-MB in the early diagnosis of acute myocardial infarction and prediction of infarcted area.

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This study was carried out on patients of a coronary unit to evaluate the diagnostic efficiency of total CPK and CPK-MB by using different analytical techniques: catalytic, immunoassisted, cellulose acetate electrophoresis, radioimmunoassay and immunoradiometric assay. The behaviour of the enzyme

Activity of phosphoglycerate mutase and its isoenzymes in serum after acute myocardial infarction.

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Aims/background-In humans there are three phosphoglycerate mutase (PGM, EC 5.4.12.1) isoenzymes (MM, MB and BB) which have similar distribution and developmental pathways to creatine kinase (CK, EC 2.7.3.2) isoenzymes. Total serum PGM activity increases in acute myocardial infarction with the same

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