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charybdotoxin/sarcoma
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3 results
Profound differences in potassium current properties of normal and Rous sarcoma virus-transformed chicken embryo fibroblasts.
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The membrane currents of chicken embryo fibroblasts (CEFs) transformed by Rous sarcoma virus (RSV) were compared with the currents of their nontransformed counterparts by using the whole-cell patch-clamp technique. In nontransformed CEFs, the main membrane current is a delayed outward K+ current
Potassium single-channel properties in normal and Rous sarcoma virus-transformed chicken embryo fibroblasts.
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Ion channels in normal and Rous sarcoma virus (RSV)-transformed chicken embryo fibroblasts (CEFs) were examined by using the patch-clamp technique. Three different types of ion channels were observed with single-channel conductances in symmetrical 140 mM KCl (with frequencies of occurrence in
Antisense knock out of the inositol 1,3,4,5-tetrakisphosphate receptor GAP1(IP4BP) in the human erythroleukemia cell line leads to the appearance of intermediate conductance K(Ca) channels that hyperpolarize the membrane and enhance calcium influx.
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To study the role of the inositol 1,3,4,5-trisphosphate-binding protein GAP1(IP4BP) in store-operated Ca2+ entry, we established a human erythroleukemia (HEL) cell line in which the expression of GAP1(IP4BP) was substantially reduced by transfection with a vector containing antisense DNA under
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