clivia/lectin

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Molecular cloning of a new lectin gene from Z. grandiflora.

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Using RNA extracted from Zephyrathes grandiflora young leaves and primers designed according to the conservative regions of Amaryllidaceae lectins, the full-length cDNA of Z. grandiflora agglutinin (ZGA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA of ZGA was 934 bp

Molecular cloning of mannose-binding lectins from Clivia miniata.

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Screening of a cDNA library constructed from total RNA isolated from young developing ovaries of Clivia miniata Regel with the amaryllis lectin cDNA clone resulted in the isolation of four different isolectin clones which clearly differ from each other in their nucleotide sequences and hence also in

[Molecular cloning and analysis of a monocot mannose-binding agglutinin from Zephyranthes grandiflora (family Amaryllidaceae)].

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The monocot mannose-binding lectin can inhibit HIV from infecting the target cells. The total RNA of Zephyranthes grandiflora was extracted and reversely transcribed into cDNA. Degenerate primers were designed based on the conserved regions of other monocot mannose-binding agglutinins by homology

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