duboisia myoporoides/scopolamine

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Decreased scopolamine yield in field-grown Duboisia plants regenerated from hairy roots.

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Hairy root cultures were obtained from hybrid clones of Duboisia myoporoides x D. leichhardtii following transformation by Agrobacterium rhizogenes strain A4. Shoots spontaneously regenerating from the hairy root cultures were rooted and transferred to soil. The plants displayed typical

Epoxidation in vivo of hyoscyamine to scopolamine does not involve a dehydration step.

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Hyoscyamine is epoxidized to scopolamine via 6beta-hydroxyhyoscyamine in several solanaceous plants. 6,7-Dehydrohyoscyamine has been proposed to be an intermediate in the conversion of 6beta-hydroxyhyoscyamine to scopolamine on the basis of the observation that this unsaturated alkaloid is converted

Winter cherry bugs feed on plant tropane alkaloids and de-epoxidize scopolamine to atropine.

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The winter cherry bug colonizes the Duboisia leichhardtii tree, which is a rich source of scopolamine. It consumes the tropane alkaloids atropine and scopolamine. Quantitative analysis revealed that the ratio of scopolamine to atropine in the winter cherry bug (0.46) was far from that found in the

Influence of Light, Temperature, and Macronutrients on Growth and Scopolamine Biosynthesis in Duboisia species.

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Scopolamine is used in the pharmaceutical industry as a precursor in the organic synthesis of different classes of important active substances and is extracted in large scale from field grown Duboisia plants. Previous research revealed that plant growth as well as production of scopolamine and its

Localization And Organization Of Scopolamine Biosynthesis In Duboisia myoporoides R. Br.

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Tropane alkaloids (TAs), especially hyoscyamine and scopolamine, are important precursors for anticholinergic and antispasmodic drugs. Hyoscyamine and scopolamine are currently obtained at commercial scale from hybrid crosses of Duboisia myoporoides × Duboisia leichhardtii plants. In this study, we

Silencing of quinolinic acid phosphoribosyl transferase (QPT) gene for enhanced production of scopolamine in hairy root culture of Duboisia leichhardtii.

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Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the form of an anesthetic and antispasmodic drug. The present study demonstrates enhanced scopolamine production from transgenic hairy root clones of Duboisia leichhardtii wherein the expression of

Change of Alkaloid Distribution in the Regenerated Plants of Duboisia myoporoides During Development.

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The alkaloid composition of regenerated plants from DUBOISIA MYOPOROIDES R. Br. (Solanaceae) callus was determined at various stages of development. Anabasine, nornicotine, scopolamine and hyoscyamine, which are main alkaloids in leaves of the mother plant, were analysed. The main alkaloids were not

Effects of Exogenous Polyamines on Tropane Alkaloid Production by a Root Culture of Duboisia myoporoides.

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The addition of putrescine, a precursor of tropane alkaloids, to the culture medium increased scopolamine production of DUBOISIA MYOPOROIDES R. Br. (Solanaceae) roots (RB-1), whereas, other precursors (ornithine, arginine, and tropine) increased the content of scopolamine only slightly or not at

Tropane alkaloid production and shoot regeneration in hairy and adventitious root cultures of Duboisia myoporoides-D. leichhardtii hybrid.

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Co-culture conditions for Duboisia myoporoides-D. leichhardtii hybrid hairy root induction were investigated using leaf explants and Agrobacterium rhizogenes ATCC 15834. The bacteria density and duration of co-culture greatly affected the induction rate; the highest rate of 50% was obtained when the

Production of tropane alkaloids by cultured cells of a Duboisia hybrid.

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The production of scopolamine-rich calli was investigated by the quantification of tropane alkaloids in a gas chromatograph equipped with a flame thermoionic detector (FTD). Stem and leaf segments from a selected strain, M-II-8-6, a hybrid between Duboisia myoporoides R. Br. and D. leichhardtii F.

Tropane alkaloid production in cultured cells of Duboisia leichhardtii.

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Calluses were derived from explants of aerial parts of a Duboisia leichhardtii F. Muell. plant. After 3 months of culture we detected small amounts of hyoscyamine and scopolamine in the unorganized calluses, but the amounts decreased until they were undetectable with successive subculture. When

Analysis of Duboisia myoporoides R. Br. and Duboisia leichhardtii F. Muell.

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Duboisia samples were analyzed for scopolamine and hyoscyamine using GLC, by a slope ratio method, and by methods employing homatropine, tetraphenylethylene, and phenylacetyltropine as internal standards. Limits of precision were determined by the inverse prediction method. Phenylacetyltropine was

Discrimination of wild types and hybrids of Duboisia myoporoides and Duboisia leichhardtii at different growth stages using 1H NMR-based metabolite profiling and tropane alkaloids-targeted HPLC-MS analysis.

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Duboisia species, which belong to the family of Solanaceae, are commercially cultivated in large scale, as they are main source of the pharmaceutically-used active compound scopolamine. In this study, 1H NMR-based metabolite profiling linking primary with secondary metabolism and additional

Tropane alkaloid production by shoot culture of Duboisia myoporoides R. Br.

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This work demonstrates the presence of hyoscyamine and scopolamine at different stages of shoot regeneration from non-organogenic and organogenic calli. The 11-week-old non-organogenic calli contained 0.41+/-0.03 and 0.23+/-0.02 microg g(-1) dry wt hyoscyamine and scopolamine respectively. However,

Alkaloid production in Duboisia hybrid hairy root cultures overexpressing the pmt gene.

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Putrescine:SAM N-methyltransferase (PMT) catalyses the N-methylation of the diamine putrescine to form N-methylputrescine, the first specific precursor of both tropane and pyridine-type alkaloids, which are present together in the roots of Duboisia plants. The pmt gene of Nicotiana tabacum was

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