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fucoidan/arachis hypogaea
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Fluorescence-labeled fucolectins are superior markers for flow cytometric quantitation of the human sperm acrosome reaction.
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OBJECTIVE
To evaluate the binding of three fluorescein (FITC)-labeled fucose-specific lectins, Anguilla anguilla agglutinin (AAA), Tetragonolobus purpureas agglutinin (TPA), and Ulex europaeus-1 agglutinin (UEA-1), to unfixed, acrosome-intact and acrosome-reacted (AR) human sperm by flow cytometry
A flow cytometric technique using peanut agglutinin for evaluating acrosomal loss from human spermatozoa.
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Flow cytometric methods for the quantification of acrosome-reacted ejaculated human spermatozoa are described in which fluorescence-labeled peanut agglutinin (Arachis hypogaea) binding to the outer acrosomal membrane is used after incubation with ethidium homodimer (EHD) as vital dye and membrane
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