glucoside/glycine max

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A novel soybean (Glycine max) gene encoding a family 3 β-glucosidase has high isoflavone 7-O-glucoside-hydrolyzing activity in transgenic rice.

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A previous study demonstrated that purified Glycine max β-glucosidase (GmBGL) could hydrolyze glucosyl isoflavone to the aglyconic form. This study reports the cloning and functional characterization of a soybean cDNA encoding the β-glucosidase. GmBGL was isolated by use of a purified soybean

cDNA cloning of a BAHD acyltransferase from soybean (Glycine max): isoflavone 7-O-glucoside-6''-O-malonyltransferase.

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A cDNA from soybean (Glycine max (L.) Merr.), GmIF7MaT, encoding malonyl-CoA:isoflavone 7-O-glucoside-6''-O-malonyltransferase, was cloned and characterized. Soybeans produce large amounts of isoflavones, which primarily accumulate in the form of their 7-O-(6''-O-malonyl-beta-D-glucosides). The cDNA

β-Sitosteryl (6'-O-linoleoyl)-glucoside of soybean (Glycine max L.) crude extract inhibits Y-family DNA polymerases.

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In the screening of selective DNA polymerase (pol) inhibitors, we isolated an acylated steryl glycoside, β-sitosteryl (6'-O-linoleoyl)-glucoside (compound 1), from the waste extract of soybean (Glycine max L.) oil. This compound exhibited a marked ability to inhibit the activities of eukaryotic

Isolation of a reduced form of cyanidin 3-O-β-D-glucoside from immature black soybean (Glycine max (L.) Merr.) and its reducing properties.

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5,7,3',4'-Tetrahydroxyflav-2-en-3-ol 3-O-β-D-glucoside was isolated from the seed coats of immature black soybeans (Glycine max (L.) Merr.). This compound is a reduced form of cyanidin 3-O-β-D-glucoside (cyanidin 3-G) which was obtained by reaction with hydrochloric acid. The molecule has reducing

Cyanidin 3-O-beta-D-glucoside isolated from skin of black Glycine max and other anthocyanins isolated from skin of red grape induce apoptosis in human lymphoid leukemia Molt 4B cells.

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Cyanidin 3-O-beta-D-glucoside (CG) was purified from black bean seed coat and other anthocyanins were prepared from red grape skin. These anthocyanins were identified by Mass, and 1H- and 13C-NMR. The effects of four anthocyanins on cell viability in human leukemia Molt 4B cells were investigated.

Isoflavone Malonyltransferases GmIMaT1 and GmIMaT3 Differently Modify Isoflavone Glucosides in Soybean (Glycine max) under Various Stresses.

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Malonylated isoflavones are the major forms of isoflavonoids in soybean plants, the genes responsible for their biosyntheses are not well understood, nor their physiological functions. Here we report a new benzylalcohol O-acetyltransferase, anthocyanin O-hydroxycinnamoyltransferase, anthranilate

Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.).

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Anthocyanin pigments in seed coats of black soybean (Glycine max (L.) Merr.) were extracted with 1% HCl-CH(3)OH, and the crude anthocyanin extract was purified by Shepadex LH-20 and Lichroprep RP-18 open-column chromatography. Three major anthocyanins were isolated, and their chemical structures

Phytochemicals and Antioxidant Activity of Korean Black Soybean (Glycine max L.) Landraces.

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Black soybean (Glycine max L.) has been used as a traditional medicine because its seed coat contains various natural phenolic compounds such as anthocyanins. The objective of this study was to reveal the genetic variation in the agricultural traits, phytochemicals, and antioxidant activity

Effect of soaking and fermentation on content of phenolic compounds of soybean (Glycine max cv. Merit) and mung beans (Vigna radiata [L] Wilczek).

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Mung beans (Vigna radiata [L] Wilczek) purchased from a Spanish company as "green soybeans", showed a different phenolic composition than yellow soybeans (Glycine max cv. Merit). Isoflavones were predominant in yellow soybeans, whereas they were completely absent in the green seeds on which

Solubilization of functional plasma membrane-localized hepta-beta-glucoside elicitor-binding proteins from soybean.

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Total membranes prepared from roots of soybean (Glycine max L.) seedlings have previously been shown to contain proteinaceous binding site(s) for a hepta-beta-glucoside elicitor of phytoalexin accumulation. The hepta-beta-glucoside elicitor-binding proteins have now been shown to co-migrate with a

Transcription analyses of GmICHG, a gene coding for a β-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr.

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Isoflavone conjugate-hydrolyzing β-glucosidase (GmICHG) of soybeans [Glycine max (L.) Merr.] catalyzes the specific hydrolysis of isoflavone conjugates (β-7-O-(malonyl)glucosides of isoflavones) to produce free isoflavones. In this study, changes in the transcription levels of GmICHG in the

Glucosylation of phosphorylpolyisoprenol and sterol at the plasma membrane of soya-bean (Glycine max) protoplasts.

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Protoplasts were prepared from cells of soya-bean (Glycine max) suspension cultures and the plasma membrane was labelled with diazotized [G-3H]sulphanilic acid. Homogenates were fractionated by differential and isopycnic centrifugation, and membrane fractions in a density gradient were characterized

Metabolism of 2,4-dichlorophenoxyacetic acid in cell suspension cultures of soybean (Glycine max L.) and wheat (Triticum aestivum L.) : I. General results.

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The metabolism of [2-(14)C]- and [ring-U-(14)C]2,4-dichlorophenoxyacetic acid (2,4-D) has been studied in cell suspension cultures of soybean (Glycine max L.) and wheat (Triticum aestivum L.). 2,4-D was extensively metabolized by both cultures, the rates of metabolism and the metabolite patterns

Quantification of cytokinin o-glucosides by negative ion mass spectrometry.

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Pulsed positive ion-negative ion chemical ionization mass spectra of O-glucosyl-zeatin and -dihydrozeatin, their ribosides, and their N-9 2-cyanoethyl and 2-chloro-2-cyanoethyl derivatives are reported. By methods based on these spectra, the levels of the glucosides were determined in soybean

Biosynthesis of flavone C-glucosides in engineered Escherichia coli.

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Two plant-originated C-glucosyltransferases (CGTs) UGT708D1 from Glycine max and GtUF6CGT1 from Gentiana triflora were accessed for glucosylation of selected flavones chrysin and luteolin. Uridine diphosphate (UDP)-glucose pool was enhanced in Escherichia coli cell cytosol by introducing

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