In order to examine the role of glutaminase in Rhizobium etli, we isolated and characterized a R. etli glutaminase mutant (LM16). This mutant was selected for its impaired ability to grow on glutamine as nitrogen and carbon source while retaining the ability to grow on other nitrogen and carbon
L-asparaginase from bacteria has been used in treatment of acute lymphoblastic leukemia. The aim of this study was to purify and characterize L-asparaginase from Phaseolus vulgaris seeds instead of microbial sources. L-asparaginase was purified to apparent homogeneity. The enzyme has molecular mass
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