Observational study of CTC microemboli in portal vein blood samples.

Pancreatic Adenocarcinoma

During Endoscopic UltraSound guided-Fine Needle Aspitation (EUS-FNA) a curvilinear echoendoscope is advanced to the distal stomach or duodenal bulb to provide a window of access to a branch of the PV. After verifying venous flow by Doppler signal, a 19-gauge EUS-FNA needle is advanced transhepatically into the portal vein branch. With the needle in the portal vein, the stylet is removed and negative-pressure suction is applied to aspirate blood. A transhepatic approach for portal vein branch access is an absolute requirement of this technique in order to minimize the risk of bleeding. The puncture site is monitored under EUS for complications.

Portal vein sampling

Manchester University NHS Foundation Trust

The Christie NHS Foundation Trust

PDAC Peripheral and Portal Vein Sampling

A Research Protocol for Collection of Peripheral and Portal Vein Blood From Patients With Pancreatic Adenocarcinoma for Translational Research

To prospectively collect paired peripheral and portal vein serum, plasma and whole blood samples for translational research at various time points. To investigate the presence of Circulating Tumour Cells and Circulating Tumour Microemboli and their biological significance. To investigate potential prognostic and predictive blood-borne genetic, protein and/or messenger ribonucleic acid biomarkers using the collected specimens.

To prospectively collect progression free and overall survival data so that correlation between biomarker parameters and clinical parameters can be examined.

Investigation of potential predictive blood germline genetic biomarkers using the collected specimens.

Identifiable personal data (IPD) will be reviewed by the investigators listed on the trial delegation log at the stage of reviewing the medical records during the hepatopancreatobiliary (HPB) MultiDisciplinary Team (MDT) meeting at Manchester University Foundation Trust where the recruitment process will start. The medical notes will also be reviewed to correlate biomarker research with clinical outcomes. However, no participants' identifiable information will be shared with anyone outside the clinical or research team.

hemothorax/carbohydrate

PDAC Peripheral and Portal Vein Sampling

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