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Page 1 from 39 results
Promotion of nicotine biosynthesis in transgenic tobacco by overexpressing allene oxide cyclase from Hyoscyamus niger.
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Plant secondary metabolites are a wide variety of low-molecular weight compounds whose productions are often enhanced in response to both biotic and abiotic stresses. Many of the responses are mediated by a class of hormones, named as jasmonates. In jasmonate biosynthetic pathway of plants, allene
Hyoscyamus muticus + Nicotiana tabacum fusion hybrids selected via auxotroph complementation.
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Protoplasts of the nicotinamide-deficient Hyoscyamus muticus cell line nic(-) IVH2 and of the nitrate reductase-deficient Nicotiana tabacum cell line NR(-) cnx 68 were induced to fuse. Selection for putative interspecific hybrid clones was via auxotroph complementation. Controls included tests for
New CMS-associated phenotypes in cybrids Nicotiana tabacum L. (+Hyoscyamus niger L.).
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Morphological characteristics were studied in cytoplasmic male sterile (CMS) cybrids possessing the tobacco nuclear genome, Hyoscyamus niger plastome and recombinant mitochondria. After backcrosses with tobacco, new flower modifications were found, including: conversions of stamens into branched
Stereochemistry and deuterium isotope effects associated with the cyclization-rearrangements catalyzed by tobacco epiaristolochene and hyoscyamus premnaspirodiene synthases, and the chimeric CH4 hybrid cyclase.
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Tobacco epiaristolochene and hyoscyamus premnaspirodiene synthases (TEAS and HPS) catalyze the cyclizations and rearrangements of (E,E)-farnesyl diphosphate (FPP) to the corresponding bicyclic sesquiterpene hydrocarbons. The complex mechanism proceeds through a tightly bound (R)-germacrene A
Extensive developmental and metabolic alterations in cybrids Nicotiana tabacum (+ Hyoscyamus niger) are caused by complex nucleo-cytoplasmic incompatibility.
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The genetic basis of multiple phenotypic alterations was studied in cell-engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of
In vitro hybridization by sexual methods and by fusion of somatic protoplasts : Experiments with Nicotiana tabacum x Petunia hybrida, N. tabacum x Hyoscyamus niger, H. niger x P. hybrida, Melandrium album x P. hybrida.
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1. No hybrid plants of Nicotiana tabacum + Petunia hybrida were regenerated from calluses of fusion experiments with mesophyll protoplasts of N. tabacum s, s (2) and v and of P. hybrida mu 1 (2). 2. After in vitro pollination of ovules of N. tabacum with pollen of P. hybrida, filamentous proembryos
Self-fertile cybrids Nicotiana tabacum (+ Hyoscyamus aureus) with a nucleo-plastome incompatibility.
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Cytoplasmic hybrids (cybrids) in a novel inter-generic combination, Nicotiana tabacum (+ Hyoscyamus aureus), were generated by fusion of protoplasts from a plastome tobacco albino mutant (line R100a1) and gamma-irradiated green protoplasts of H. aureus. Cybrids possessed a plastome of H. aureus and
FAILURE OF ANAESTHETIZED APHIDS TO ACQUIRE OR TRANSMIT HENBANE MOSAIC VIRUS WHEN THEIR STYLETS WERE ARTIFICIALLY INSERTED INTO LEAVES OF INFECTED OR HEALTHY TOBACCO PLANTS.
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Observations on the Chemical and Physiological Properties of the Empyreumatic Oils of Foxglove, Henbane, and Tobacco.
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Metabolic characterization of Hyoscyamus niger root-specific putrescine N-methyltransferase.
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N-methylputrescine is the precursor of nicotine and pharmaceutical tropane alkaloids such as hyoscyamine. Putrescine N-methyltransferase (PMT) catalyzes the N-methylation of putrescine to form N-methylputrescine. While the role of PMT in nicotine biosynthesis is clear, knowledge of PMT in the
A soluble auxin-binding protein from Hyoscyamus muticus is a glutathione S-transferase.
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We have used the photoaffinity label azido-[3H]IAA (5-N3-[7-3H]indole-3-acetic acid), a biologically active analog of indole-3-acetic acid, to identify auxin-binding proteins (ABPs) in the soluble fraction of Hyoscyamus muticus. A 25-kD polypeptide previously described (H. Macdonald, A. M. Jones, P.
Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures.
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Hyoscyamine-6beta-hydroxylase (H6H) catalyses the conversion of hyoscyamine into its epoxide scopolamine, a compound with a higher added value in the pharmaceutical market than hyoscyamine. We report the establishment of tobacco cell cultures carrying the Hyoscyamus muticus h6h gene under the
Intraspecific variability of the tandem repeats in Nicotiana putrescine N-methyltransferases.
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The putrescine N-methyltransferase (PMT) cDNA clone previously isolated from tobacco encodes a spermidine synthase-like protein with an 11 amino acid element repeated four times in tandem at the amino terminus. Genomic Southern blot analyses indicated that this N-terminal repeat array is found in
Putrescine and putrescine N-methyltransferase in the biosynthesis of tropane alkaloids in cultured roots of Hyoscyamus albus : I. Biochemical studies.
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The activity of arginine decarboxylase (EC 4.1.1.19) in cultured roots of Hyoscyamus albus L., which produce considerable amounts of tropane alkaloids, was twice that of ornithine decarboxylase (EC 4.1.1.17), both activities being highest during active root growth, whereas arginase (EC 3.5.3.1)
Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases.
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Genomic and cDNA clones for vetispiradiene synthase, a sesquiterpene cyclase found in Hyoscyamus muticus, were isolated using a combination of reverse transcription-polymerase chain reactions and conventional cloning procedures. RNA blot hybridization demonstrated an induction of mRNA consistent
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