1. Lysolecithin, prepared by the action of snake-venom phospholipase A on ovolecithin, when incubated with Savoy-cabbage phospholipase D, in the presence of Ca(2+) ions, gave two degradation products (designated A and B) in the form of their calcium salts. 2. These calcium salts were separated
1. The action of the water-soluble phospholipase D from Savoy cabbage leaves upon an aqueous solution of lysolecithin has been studied. 2. Optimum conditions required the presence of Ca(2+) ions and pH5.8. 3. Equivalence was found between the amounts of lysolecithin hydrolysed and free choline
An 9.4-kDa antifungal peptide designated as campesin was isolated from seeds of the cabbage Brassica campestris. The isolation procedure involved affinity chromatography on Affi-gel blue gel, ion exchange chromatography on Q-Sepharose and Mono S, and gel filtration on Superdex 75 and Superdex
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