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3 results
Triterpene cyclases from Oryza sativa L.: cycloartenol, parkeol and achilleol B synthases.
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The gene products of AK121211, AK066327, and AK070534 from Oryza sativa encode cycloartenol, parkeol, and achilleol B synthases, respectively. Parkeol synthase is a unique enzyme that affords parkeol as a single product. Achilleol B synthase is the third seco-type triterpene cyclase identified to
Oryza sativa parkeol cyclase. Changes in the substrate folding conformation and the deprotonation sites by mutations at Tyr257: Importance of the hydroxyl group and steric bulk.
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The Y257 of Oryza sativa parkeol synthase (OsOSC2) corresponds to the H234 of S. cerevisiae lanosterol cyclase (ScLAS), which is proposed to be responsible for the final deprotonation reaction. The Ala mutant afforded nine tetracyclic skeletons as the main products, which consisted of protostadieol
Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species.
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Triterpene synthases, also known as 2,3-oxidosqualene cyclases (OSCs), synthesize diverse triterpene skeletons that form the basis of an array of functionally divergent steroids and triterpenoids. Tetracyclic and pentacyclic triterpene skeletons are synthesized via protosteryl and dammarenyl
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