Upon dehardening of stem bark of black locust (Robinia pseudoacacia), a significant decrease in phospholipid content on a milligram protein basis was observed in various crude particulate cell fractions. To ascertain this with a defined membrane, microsomal preparations were separated into several
Freeze-thawing of microsome-enriched membranes from living bark tissues of black locust trees, especially those from less hardy tissues, caused a drastic increase in sensitivity to Ca(2+) and a complete loss of the regulatory action of Mg(2+) in membrane-bound phospholipase D activity with
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