Select the individual:
Healthy, non-pregnant adults 18-65 years of age, not taking any medication, including NSAIDs, not taking antibiotics within 4 weeks of study, BMI within 18-35 range and stable weight.
Exclusion criteria: children and elderly (over 65), pregnancy, diagnosed with non-communicable or communicable disease, being under restrictive diet, antibiotics within 4 weeks of study, medications within 4 weeks of study. They will eat beans for 5 days. The beans will be soaked for 3 h, water discarded and beans cooked for 20 min in pressure cook. And in the end for the week, adults will be eat the bean enrichment of deuterium for availability protein, collected urine, saliva and blood.

Fifty children will be recruited from ages varying from 1 to 3 years and of both sexes. Children who use medications, who do not habitually consume beans, will be excluded and when there is no explicit written authorization from the parents or guardians.
All the children with different status of nutrition will be eaten the beans. They will eat beans for 5 days. The beans will be soaked for 3 h, water discarded and beans cooked for 20 min in pressure cook. And in the end for the week, children will be eat the bean enrichment of deuterium for availability protein, collected urine or saliva depend on the best biological samples of pilot study (adult).

Fifty individuals of both sexes will be recruited, previously evaluated by complete clinical / laboratory examination and medical history. The elderly will be excluded from antiinflammatory, chemotherapeutic, corticoid, allergy and bean aversion or antibiotic therapy. These drugs could interfere with protein bioavailability. They will eat beans for 5 days. The beans will be soaked for 3 h, water discarded and beans cooked for 20 min in pressure cook. And in the end for the week, elderly will be eat the bean enrichment of deuterium for availability protein, collected urine or saliva depend on the best biological samples of pilot study (adult).

Protein Malabsorption

Nutrition Disorders

Sarcopenia

Bioavailability of Proteins From Plant Based Diets

Bioavailability of Proteins From Beans Usually Consumed in the Brazilian Diet (Phaseolus Vulgaris) According to Different Preparation Techniques.

Il will be measured new stable isotope method using deuterium for the study of the bioavailability of protein derived from beans in healthy adults, children and elderly. It will be assessed 45 persons, express in delta enrichment and analysis in Mass Spectrometry

Il will be measured stable isotope using Carbon 13 for the study of the bioavailability of protein derived from beans in healthy adults, children and elderly. It will be assessed 45 persons, express in delta enrichment and analysis in Mass Spectrometry

3 samples for beans cooked will be collected and will be verified the nutrients composition properties of the usually consumed Brazilian bean (Phaseolus vulgaris) labelled with deuterium like macronutrients and micronutrients in grams.

Urinary nitrogen of the samples will be analysed by the Kjeldahl method with ammonium micro diffusion

There is no plan to make this data available to other researchers. We will publish this data in the scientific magazine

sarcopenia/phaseolus vulgaris

Bioavailability of Proteins From Plant Based Diets

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