The structure of the plasmalemma of isolated protoplasts from stem callus cultures of Skimmia japonica was investigated during cell wall regeneration by means of freeze-etching and deep-etching. Three of the four theoretically possible views of the membrane-the two corresponding inner fracture faces
Isolated protoplasts obtained from leaves and from stem callus cultures of Skimmia japonica were cultivated for 72 h to regenerate a new cell wall. During this process the structural changes in the protoplasts and at the surface of the plasmalemma were studied in ultrathin sections and after
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