smooth hawksbeard/agar

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Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species.

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Two novel techniques improve division and colony formation from protoplasts: 1) Plating in agarose stimulates colony formation of protoplasts from a wide range of species. Protoplasts from Nicotiana tabacum developed to colonies from lower initial population densities in agarose than in agar or

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