soybean lectin/hypersensitivity

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Lectin-binding ability of immunoglobulin E and its participation in triggering of mast cells.

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Eleven purified lectins were compared in their IgE-precipitating, mast-cell-degranulating and histamine-releasing activity and in eliciting skin allergic reactions in atopic or normal people. A significantly positive relationship could be proved between their IgE-precipitating activity and

Non-specific binding of anti-human IgE peroxidase-linked conjugates to legume proteins in immunoblots.

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Non-specific binding was observed in immunoblotting of legume proteins with commercial anti-human IgE and IgG antibodies conjugated with peroxidase when incubations were conducted without prior incubation with human sera of legume-allergic individuals. The non-specific binding was encountered with

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