Hydroponically grown tobacco (Nicotiana tabacum L. cv. Samsun) roots exude proteases under non-stressed conditions. Ten different proteases could be distinguished by 2D-zymography of root exudate. The majority of the gelatinolytic activity was susceptible to serine protease inhibitors. One of the
Plasma membrane-associated proteases (pm-proteases) exist principally in roots of Nicotiana tabacum cv. Samsun, whereas in plasma membrane (pm) vesicles prepared from leaves, protease activity was at the detection limit. Biochemical characterisation revealed a high diversity of particular
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