FEBS Letters 1988-Oct
Two conserved tryptophan residues of tumor necrosis factor and lymphotoxin are not involved in the biological activity.
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Abstracto
Each of the two highly conserved tryptophan residues in hTNF (positions 28 and 114) was converted into phenylalanine by site-directed mutagenesis and the mutant proteins were partially purified. A cytotoxicity assay on mouse L929 cells showed only a slight reduction in biological activity, strongly suggesting that neither of the two amino acids is involved in the active site.
