International Journal of Clinical and Experimental Pathology 2017

Lycium barbarum polysaccharide inhibits gastric cancer cell proliferation, migration and invasion by down-regulation of MMPs and suppressing epithelial-mesenchymal transition.

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Qian Chen

Rongliang Shi

Daowen Jiang

Weiyan Liu

Zhenyi Jia

ARTÍCULO: 31966578

BioSeek: 31966578

Palabras clave

Abstracto

OBJECTIVE

To evaluate the effect of Lycium barbarum polysaccharide (LBP) on gastric cancer (GC) cells and to explore the associated mechanism.

Human GC SGC-7901 cells were divided into control, 10 μM LBP, 20 μM LBP and 50 μM LBP groups. CCK8 assay and Transwell assay were performed to evaluate the proliferation, migration and invasion of SGC-7901 cells. Western blotting was used to determine protein expressions.The proliferation, migration and invasion decreased significantly in 20 μM LBP and 50 μM LBP groups. As the result of Western blotting, protein levels of MMP2, MMP9, Snail and vimentin decreased in 20 μM LBP and 50 μM LBP groups with different degrees. The expression E-cadherin significantly increased in all three experimental groups. The phosphorylation levels of AKT and PI3K in 20 μM LBP and 50 μM LBP groups were much lower than control group.LBP could inhibit the proliferation, migration and invasion of human GC cells bydown-regulation of MMPs and suppression of epithelial-mesenchymal transition (EMT).

Lycium barbarum polysaccharide inhibits gastric cancer cell proliferation, migration and invasion by down-regulation of MMPs and suppressing epithelial-mesenchymal transition.

Palabras clave

polisacárido

cáncer

lycium

lycium barbarum

Abstracto

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