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anisodus sinensis/alkaloid
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Effects of different elicitors on yield of tropane alkaloids in hairy roots of Anisodus acutangulus.
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The four tropane alkaloids have played a pivotal role in controlling diseases such as the toxic and septic shock, the organophosphorus poison and the acute lung injury. Here, the elicitation effect of different elicitors on the production of tropane alkaloids and the molecular mechanism of enzyme
Molecular cloning and characterization of two tropinone reductases in Anisodus acutangulus and enhancement of tropane alkaloid production in AaTRI-transformed hairy roots.
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Tropane alkaloids are used medicinally as anticholinergic agents with increasing market demand, so the improvement and production of active components from medicinal plants using molecular biotechnology show great potential for applications that should benefit human healthcare. Two tropinone
Enhancing Tropane Alkaloid Production Based on the Functional Identification of Tropine-Forming Reductase in Scopolia lurida, a Tibetan Medicinal Plant.
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Scopolia lurida, a native herbal plant species in Tibet, is one of the most effective producers of tropane alkaloids. However, the tropane alkaloid biosynthesis in this plant species of interest has yet to be studied at the molecular, biochemical, and biotechnological level. Here, we report on the
Transcriptome exploration for further understanding of the tropane alkaloids biosynthesis in Anisodus acutangulus.
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Tropane alkaloids (TAs) such as anisodamine, anisodine, hyoscyamine and scopolamine are extensively used in clinical practice as anticholinergic agents. Anisodus acutangulus produces TAs in root tissue, and although several genes involved in scopolamine biosynthesis have been cloned, yet the
Co-expression of AaPMT and AaTRI effectively enhances the yields of tropane alkaloids in Anisodus acutangulus hairy roots.
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BACKGROUND
Tropane alkaloids (TA) including anisodamine, anisodine, hyoscyamine and scopolamine are a group of important anticholinergic drugs with rapidly increasing market demand, so it is significant to improve TA production by biotechnological approaches. Putrescine N-methyltransferase (PMT) was
Enhancing the production of tropane alkaloids in transgenic Anisodus acutangulus hairy root cultures by over-expressing tropinone reductase I and hyoscyamine-6β-hydroxylase.
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Tropane alkaloids (TA) including hyoscyamine, anisodamine, scopolamine and anisodine, are used medicinally as anticholinergic agents with increasing market demand, so it is very important to improve TA production by metabolic engineering strategy. Here, we report the simultaneous introduction of
[Mydriatic alkaloid content of Scopolia lurida].
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[Studies on alkaloids of Scopolia lurida Dun. Assays of atropine, scopolamine and cuscohygrine].
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[Pharmacological study of an alkaloid extracted from the roots of Anisodus luridus (Himalayan Scopolia)].
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Comparison of two hyoscyamine 6β-hydroxylases in engineering scopolamine biosynthesis in root cultures of Scopolia lurida.
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Scopolia lurida, a medicinal plant native to the Tibetan Plateau, is among the most effective producers of pharmaceutical tropane alkaloids (TAs). The hyoscyamine 6β-hydroxylase genes of Hyoscyamus niger (HnH6H) and S. lurida (SlH6H) were cloned and respectively overexpressed in hairy root cultures
Efficient biosynthesis of rare natural product scopolamine using E. coli cells expressing a S14P/K97A mutant of hyoscyamine 6β-hydroxylase AaH6H.
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Hyoscyamine 6β-hydroxylase (H6H, EC 1.14.11.11), an α-ketoglutarate dependent dioxygenase catalyzes the hydroxylation of (-)-hyoscyamine and the subsequent epoxidation of 6β-hydroxyhyoscyamine to form scopolamine, a valuable natural alkaloid. In this study, random mutagenesis and site-directed
De Novo Production of the Plant-Derived Tropine and Pseudotropine in Yeast.
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Tropine and pseudotropine with opposite stereospecific configurations as platform compounds are central building blocks in both biosynthesis and chemical synthesis of pharmacologically important tropane and nortropane alkaloids. The supply of plant-derived tropine and pseudotropine still heavily
Molecular characterization and expression analysis of two distinct putrescine N-methyltransferases from roots of Anisodus acutangulus.
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Putrescine N-methyltransferase (PMT; EC. 2.5.1.53) catalyzes the S-adenosylmethionine-dependent N-methylation of putrescine to form N-methylputrescine, which was the first committed step in tropane alkaloid biosynthetic pathway. Two PMT cDNA clones [Anisodus acutangulus putrescine
Comparison on profiling of hairy root of two Tibetan medicinal plants Przewalskia tangutica Maxim and Anisodus tanguticus Maxim.
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Tropane alkaloids (TAs) are important drugs for curing many diseases in the medical industry. To sustainably exploit TA resources in traditional endangered Tibetan herbs, the hairy root (HR) systems of Przewalskia tangutica Maxim. and Anisodus tanguticus Maxim. were compared under the same culture
Absolute configuration of (-)anisodine (a new ganglio blocking agent) and (-)anisodinic acid.
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A new tropane alkaloid, (-)anisodine (I), was isolated from the plant of Anisodus tanguticus. It is a novel ganglio blocking agent. The absolute configuration of (-)anisodinic acid (VII), which is the side chain of (-)anisodine, was determined by chemical correlation with (R) (-)-2-phenyl-1,
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