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13 resultados
Cytidine-to-Uridine RNA editing factor NbMORF8 negatively regulates plant immunity to Phytophthora pathogens
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Mitochondria and chloroplasts play key roles in plant-pathogen interactions. Cytidine-to-uridine (C-to-U) RNA editing is a critical post-transcriptional modification in mitochondria and chloroplasts that is specific to flowering plants. Multiple organellar RNA editing factors (MORFs) form a protein
Application of Cas12a and nCas9-activation-induced cytidine deaminase for genome editing and as a non-sexual strategy to generate homozygous/multiplex edited plants in the allotetraploid genome of tobacco.
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Protoplasts can be used for genome editing using several different CRISPR systems, either separately or simultaneously, and that the resulting mutations can be recovered in regenerated non-chimaeric plants. Protoplast transfection and regeneration systems are useful platforms for CRISPR/Cas
Characterization of filament-forming CTP synthases from Arabidopsis thaliana.
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Cytidine triphosphate (CTP) is essential for DNA, RNA and phospholipid biosynthesis. De novo synthesis is catalyzed by CTP synthases (CTPS). Arabidopsis encodes five CTPS isoforms that unanimously share conserved motifs found across kingdoms, suggesting all five are functional enzymes. Whereas
Organization and transcription of the gene family encoding chlorophyll a/b-binding proteins in Nicotiana sylvestris.
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Nine Lhcb1 genes encoding the light-harvesting chlorophyll a/b-binding proteins of photosystem II were isolated and characterized from Nicotiana sylvestris. Their nucleotide sequences are highly similar. Lhcb1 transcripts are accumulated in leaves and stems but not in roots and non-green cultured
In vitro analysis of transcription initiation and termination from the Lhcb1 gene family in Nicotiana sylvestris: detection of transcription termination sites.
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Genes encoding chlorophyll a/b-binding proteins of photosystem II (Lhcb) constitute a multigene family. Nine Lhcb1 genes have previously been isolated from the tobacco species, Nicotiana sylvestris, and the transcription initiation sites in vivo have been mapped. Reaction conditions from a
Distribution of incorporated, synthetic cytokinins in ribosomal RNA preparations from tobacco callus.
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The distribution of incorporated synthetic cytokinins (N(6)-[8-(14)C]benzyladenine ([8-(14)C]bzl(6)Ade) and N(6)[8-(14)C]furfuryladenine ([8-(14)C]fr(6)Ade) in ribosomal RNA prepared from tobacco callus (Nicotiana tabacum L. var. Wis. No. 38) grown in the presence of one of these for 25 or 26 days
EVIDENCE FOR CYTOPLASMIC DNA IN ROOT CELLS OF NICOTIANA.
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Sterile root cultures from Nicotiana tabacum were grown with H(3)-thymidine added to the medium for various intervals. Incorporation of the labeled nucleoside into nuclear DNA occurred in a fraction of the nuclei which increased with time. In addition, the cytoplasm of all cells incorporated enough
Potato virus X Vector-Mediated DNA-Free genome editing in plants
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Genome editing technology is important for plant science and crop breeding. Genome-edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA, which is problematic for production of genome-edited transgene-free plants for vegetative propagation or highly heterozygous hybrid
N 6 -methyl-adenosine level in Nicotiana tabacum is associated with tobacco mosaic virus.
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N 6 -methyl-adenosine (m6A) is a prevalent RNA modification in many species. Abnormal m6A methylation levels can lead to RNA dysfunction and can cause diseases. Tobacco mosaic virus (TMV) is one of the most devastating viruses for agricultural plants. It has many hosts, particularly including
Establishment of a Heterologous RNA Editing Event in Chloroplasts.
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In chloroplasts and plant mitochondria, specific cytidines in mRNAs are post-transcriptionally converted to uridines by RNA editing. Editing sites are recognized by nucleus-encoded RNA-binding proteins of the pentatricopeptide repeat (PPR) family, which bind upstream of the editing site in a
Computational analysis of RNA editing sites in plant mitochondrial genomes reveals similar information content and a sporadic distribution of editing sites.
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A computational analysis of RNA editing sites was performed on protein-coding sequences of plant mitochondrial genomes from Arabidopsis thaliana, Beta vulgaris, Brassica napus, and Oryza sativa. The distribution of nucleotides around edited and unedited cytidines was compared in 41 nucleotide
Sequence of the tomato chloroplast DNA and evolutionary comparison of solanaceous plastid genomes.
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Tomato, Solanum lycopersicum (formerly Lycopersicon esculentum), has long been one of the classical model species of plant genetics. More recently, solanaceous species have become a model of evolutionary genomics, with several EST projects and a tomato genome project having been initiated. As a
DNA methylation, vernalization, and the initiation of flowering.
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Late-flowering ecotypes and mutants of Arabidopsis thaliana and the related crucifer Thlaspi arvense flower early after cold treatment (vernalization). Treatment with the DNA demethylating agent 5-azacytidine induced nonvernalized plants to flower significantly earlier than untreated controls.
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