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dichloromethane/triacylglycerol
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Regioselective separation of isomeric triacylglycerols by reversed-phase high-performance liquid chromatography: stationary phase and mobile phase effects.
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Complete regioselective separation of five pairs of isomeric dipalmitoyl polyalkenoyl glycerols with two to six double bonds in the unsaturated acyl residues has been achieved by RP-HPLC on a single ODS column. Four ODS columns with stationary phases containing different percentages of free silanol
Resolution of triacylglycerol positional isomers by reversed-phase high-performance liquid chromatography.
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The ability of reversed-phase high-performance liquid chromatography (RP-HPLC) to separate some positionally isomeric disaturated and monounsaturated triacylglycerols (TAGs) as intact species is demonstrated for the first time. Mobile phases of acetonitrile modified with methanol, ethanol,
Conjugated linoleic acid-rich soy oil triacylglycerol identification.
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Conjugated linoleic acid (CLA)-rich soy oil has been produced by soy oil linoleic acid (LA) photoisomerization, but CLA-rich oil triacylglycerol (TAG) characterization was not described. Therefore, the objectives were to identify and quantify new TAG fractions in CLA-rich oil by nonaqueous
Rapid analysis of triacylglycerols using high-performance liquid chromatography with light scattering detection.
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This paper details a high-performance liquid chromatography (HPLC) method for the separation of triacylglycerols, using a 3-micron, 15 cm x 4.6 mm I.D. Spherisorb ODS column and gradient elution with dichloromethane and acetonitrile. The triacylglycerols are detected using a light scattering
Analysis of human milk triacylglycerols by high-performance liquid chromatography with light-scattering detection.
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A high-performance liquid chromatography (HPLC) method for the separation of human milk triacylglycerols using a C18 Spherisorb ODS column and ternary gradient elution with dichloromethane, acetone and acetonitrile is described. The triacylglycerols are detected by light scattering. Several
Separation of triacylglycerols and free fatty acids in microalgal lipids by solid-phase extraction for separate fatty acid profiling analysis by gas chromatography.
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Microalgal lipids were separated into two fractions, triacylglycerols (TAGs) and free fatty acids (FFAs), by solid-phase extraction employing sodium carbonate as the sorbent and dichloromethane (20% by volume) in n-hexane as the extracting solvent. The TAG fraction was then saponified, followed by
Retention behavior of isomeric triacylglycerols in silver-ion HPLC: effects of mobile phase composition and temperature.
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A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver-ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40°C using the most widespread hexane- and dichloromethane-based mobile phases. The randomization of mono-acyl TG standards and the random
Determination of the triacylglycerol composition of coffee beans by reverse-phase high-performance liquid chromatography.
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Reverse-phase HPLC with refractive index and light scattering detectors in isocratic and gradient elution modes, respectively, was applied for the separation of the major triacylglycerols (TAG) in coffee lipids. Twelve TAG species could be identified and determined using a linear gradient of
Triacylglycerol composition of winged bean (Psophocarpus tetragonolobus).
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Winged beans were extracted with hexane and the extracted lipid was analyzed by HPLC for its triacylglycerol (triglyceride) composition on a Lichrosorb RP-18 column with acetonitrile-tetrahydrofuran-dichloromethane (6:2:1) solvent mixtures. The chromatography yielded more than 21 peaks, and each
[Identification of triacylglycerols in coix oil by high performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry].
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OBJECTIVE
To identify triacylglycerols in coix oil.
METHODS
High performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry was used for identification. The experiment was operated under the conditions: spray voltage at 3 000 V, capillary temperature at 250 degrees
Separation of molecular species of triacylglycerols by high-performance liquid chromatography with a silver ion column.
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Molecular species representative of the wide range of triacylglycerols occurring in nature, ranging from relatively saturated fats such as that from sheep adipose tissue through polyunsaturated seed oils, including sunflower and linseed oils, to an oligounsaturated fish oil, have been resolved by
The role of surface chemistry for the super-hydrophobicity of the springtail Orchesella cincta (Insecta:Collembola).
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Collembola are ancient arthropods living in soil with extensive exposure to dirt, bacteria and fungi. To protect from the harsh environmental conditions and to retain a layer of air for breathing when submerged in water, they have evolved a superhydrophobic, liquid repelling cuticle surface. The
Identification of lipids in the cuticle of the parasitic nematode Anisakis simplex and the somatic tissues of the Atlantic cod Gadus morhua.
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The main aim of this work was to assign the cuticular lipids identified in a parasitic nematode and to distinguish those originating from its host. The hypothesis that long-chained fatty acids and sterols are imported by the parasite in the absence of certain enzymes was also tested. The organisms
Prunus mume leaf extract lowers blood glucose level in diabetic mice.
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Context Diabetes is a common metabolic disease with long-term complications. Prunus mume Sieb. et Zucc. (Rosaceae) fruits have shown to ameliorate glucose intolerance. However, the antidiabetic effects of P. mume leaves have not been investigated. Objective This study evaluated the effects of P.
Stereoselectivity of lipases: esterification reactions of octadecylglycerol.
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Stereoselectivity of several triacylglycerol lipases (EC 3.1.1.3) has been investigated in the enzymatic esterification of rac-1-O-octadecylglycerol with oleic acid in the presence of organic solvents, such as hexane. X-1(3)-O-Octadecylmonooleoylglycerols were the only products formed with most
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