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giardiasis/carbohydrate
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Giardia cyst wall-specific carbohydrate: evidence for the presence of galactosamine.
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Gas chromatographic (GC), mass spectrometric (MS), lectin binding and enzymatic analyses of the carbohydrates from Giardia cyst walls, intact cysts and trophozoites were performed to investigate the carbohydrate composition of Giardia cyst walls and to test the hypothesis that the Giardia cyst wall
Carbohydrate and amino acid analyses of Giardia muris cysts.
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Intact Giardia muris cysts were subjected to consecutive chloroform/methanol and 2% sodium dodecyl sulfate (SDS) extractions, and to amyloglucosidase treatment. The SDS-insoluble, amyloglucosidase-fast cyst walls (ACW) were further incubated with chymotrypsin, trypsin, papain, or pronase. Low
Lipid and carbohydrate metabolism of Giardia lamblia.
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The lipid and carbohydrate metabolism of Giardia lamblia was studied using trophozoites isolated from a human and axenically grown in vitro in medium containing fetal bovine serum. The phospholipid, fatty acid and neutral lipid composition of the G. lamblia trophozoites was similar to that of the
Effects of asymptomatic Giardia intestinalis infection on carbohydrate absorption in well-nourished Mexican children.
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The aim of this study was to evaluate carbohydrate absorption in well-nourished children with asymptomatic giardiasis. Two groups were selected based on results of stool examination of 211 children attending pre-school centers in Hermosillo, Sonora, Mexico: a control group of six non-infected
A new set of carbohydrate-positive vesicles in encysting Giardia lamblia.
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Giardia lamblia is a protozoan parasite that presents both trophozoite and cyst forms. In this study, the distribution of the different sugar residues and the origin of the carbohydrate components of the cyst wall were studied using transmission electron microscopy, ultrastructural cytochemistry for
Glycogen as a carbohydrate energy reserve in trophozoites of Giardia lamblia.
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Although there is indirect evidence to suggest that glycogen is present in G. lamblia, to date it has not been purified and identified from this organism. In this study, a high molecular weight carbohydrate was purified and characterized and its physiological role as an energetic reserve was
Regulation of carbohydrate metabolism during Giardia encystment.
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Giardia intestinalis trophozoites encyst when they are exposed to bile. During encystment, events related to the inducible synthesis of a novel N-acetyl-D-galactosamine (GalNAc) homopolymer, occur. Within the first 6 h of encystment, mRNA for glucosamine 6-P isomerase (GPI), the first inducible
[Evaluation of blood insulin level and digestion and absorption of carbohydrates in children with lambliasis].
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[Disturbances in carbohydrate digestion in a case lambliasis].
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Carbohydrate and amino acid metabolism in Giardia: a review.
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[Intestinal malabsorption of carbohydrates in lambliasis. Study of 8 cases].
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Wheat germ supplement reduces cyst and trophozoite passage in people with giardiasis.
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The protozoan parasite Giardia lamblia is a major cause of waterborne enteric disease worldwide. Lectins are proteins that bind to carbohydrate (sugar) moieties. Potential targets for lectins are found on the surface of most single-celled organisms. Modest concentrations of wheat germ agglutinin
The UDP-glucose pyrophosphorylase from Giardia lamblia is redox regulated and exhibits promiscuity to use galactose-1-phosphate.
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BACKGROUND
Giardia lamblia is a pathogen of humans and other vertebrates. The synthesis of glycogen and of structural oligo and polysaccharides critically determine the parasite's capacity for survival and pathogenicity. These characteristics establish that UDP-glucose is a relevant metabolite, as
The generation gap: Proteome changes and strain variation during encystation in Giardia duodenalis.
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The prevalence of Giardia duodenalis in humans is partly owed to its direct and simple life cycle, as well as the formation of the environmentally resistant and infective cysts. Proteomic and transcriptomic studies have previously analysed the encystation process using the well-characterised
A simple fibril and lectin model for cyst walls of Entamoeba and perhaps Giardia.
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Cyst walls of Entamoeba and Giardia protect them from environmental insults, stomach acids, and intestinal proteases. Each cyst wall contains a sugar homopolymer: chitin in Entamoeba and a unique N-acetylgalactosamine (GalNAc) homopolymer in Giardia. Entamoeba cyst wall proteins include Jacob
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