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hemolysis/phosphatase
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Normal values of alkaline phosphatase, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in the serum of experimental animals using optimised methods, and the effects of haemolysis on these values.
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Normal values for alkaline phosphatase, glutamic oxaloacetic transaminase and glutamic pyruvic transaminase in rats and dogs were determined by optimised assay methods. It was also demonstrated that haemolysis caused the results to deviate considerably from the true values.
Alkaline phosphatase as an early marker of hemolysis in newborns.
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BACKGROUND
Early diagnosis and appropriate management of neonatal hyperbilirubinemia are very important in order to prevent bilirubin encephalopathy and kernicterus. Several diagnostic tests may be used for this purpose, including bilirubin level itself. The aim of the present study was to
Unusually Low Serum Alkaline Phosphatase Activity in a Patient with Acute on Chronic Liver Failure and Hemolysis.
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A 28-year-old male with acute on chronic liver failure (ACLF) and hepatic encephalopathy had deranged liver function with curiously low level (0-15 IU/L) of serum alkaline phosphatase (ALP). Peripheral smear examination suggested hemolytic anemia. The finding of persistent low ALP, after ruling out
The effect of haemolysis on the measurement of plasma alkaline phosphatase activity.
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The non-dialysable fraction of haemolysate causes an apparent reduction of plasma alkaline phosphatase (ALP) activity using 4-nitrophenylphosphate as substrate. Analyses using four different buffers showed that the decrease in enzyme activity is affected by the buffer used. The percentage reduction
Severe hemolysis and methemoglobinemia following fava beans ingestion in glucose-6-phosphatase dehydrogenase deficiency: case report and literature review.
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BACKGROUND
Reduced concentrations of glucose-6-phospate dehydrogenase (G6PD) render erythrocytes susceptible to hemolysis under conditions of oxidative stress. In favism, the ingestion of fava beans induces an oxidative stress to erythrocytes, leading to acute hemolysis.
CONCLUSIONS
The simultaneous
Unmeasurable serum alkaline phosphatase activity in Wilson's disease associated with fulminant hepatic failure and hemolysis.
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Erythrocyte acid phosphatase polymorphism and haemolysis.
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Haemolysis and plasma alkaline phosphatase activity.
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Alcohol Used as Disinfectant before Venipuncture does not Lead to Sample Haemolysis or Sample Dilution.
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BACKGROUND
Sample haemolysis is often the leading cause of sample rejections in clinical laboratory. Isopropyl alcohol or ethyl alcohol, used as disinfectant during sample collection is often considered an important cause of sample haemolysis or sample dilution; although there is a paucity of
The effect of dietary oxidized fats on the antioxidant status of erythrocytes and their susceptibility to haemolysis in rats and guinea pigs.
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Three experiments were carried out with rats (experiments 1 and 2) and guinea pigs (experiment 3) to study the effect of oxidized fats, in interaction with dietary concentrations of vitamins E and C, on the antioxidant status of erythrocytes and the rate of haemolysis. In experiment 1, diets with
[Posthepatic obstructive jaundice caused by primary extragonadal germ-cell tumor in a patient with glucose-6-phosphatase dehydrogenase deficiency].
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METHODS
In patients with glucose-6-phosphatase dehydrogenase (G6PD) deficiency (favism) jaundice is usually caused by hemolysis due to stress, infection or following the application of drugs. We report on a 74-year-old Italian with known G6PD deficiency complaining of jaundice, weight loss and
Detection of hantavirus infection in hemolyzed mouse blood using alkaline phosphatase conjugate.
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Conventional methods such as ELISA and culture require a long time for the determination of viral infections. Fast and reliable instrumentation suitable for operation under field conditions that allows rapid detection of hantavirus in rodent populations in the wild, would be a substantial
Study of a kindred with partial deficiency of red cell 2,3-diphosphoglycerate mutase (2,3-DPGM) and compensated hemolysis.
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A kindred with partial deficiency of red cell 2,3-diphosphoglycerate mutase (2,3-DPGM) was studied. The propositus presented with indirect hyperbilirubinemia, normal hemoglobin (15.8 g/dl), and elevated reticulocyte count (4.6%). The red cell 51Cr survival was decreased (tau1/2 16 days). Incubated
Predictors of severe hemolysis in patients with glucose-6-phosphate dehydrogenase deficiency following exposure to oxidant stresses.
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OBJECTIVE
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a genetic enzymatic disorder that affects millions of people worldwide, and is a major health problem in Jordan. We studied factors that may predict severe hemolysis in children with G6PD deficiency.
METHODS
We reviewed the records of
Influence of hemolysis on clinical chemistry parameters determined with Beckman Coulter tests - detection of clinically significant interference.
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The aim of this study was to examine the influence of hemolysis on 25 clinical chemistry parameters and to compare the resulting bias with clinically significant differences and the manufacturer's specifications. Using freeze-thawing of the treated blood aliquot of each subject (N = 17), four
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