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Mutation of the PAX6 gene in patients with autosomal dominant keratitis.
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Autosomal dominant keratitis (ADK) is an eye disorder chiefly characterized by corneal opacification and vascularization and by foveal hypoplasia. Aniridia (shown recently to result from mutations in the PAX6 gene) has overlapping clinical findings and a similar pattern of inheritance with ADK. On
The involvement of proline-rich protein Mus musculus predicted gene 4736 in ocular surface functions.
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OBJECTIVE
To research the two homologous predicted proline-rich protein genes, Mus musculus predicted gene 4736 (MP4) and proline-rich protein BstNI subfamily 1 (Prb1) which were significantly upregulated in cultured corneal organs when encountering fungal pathogen preparations. This study was to
Immunological studies on herpetic keratitis: effect of IL-2 on HSV-specific CTL.
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The effect of interleukin 2 (IL-2) on cytotoxic T lymphocytes (CTL) was examined in mice with corneal infection of herpes simplex virus (HSV). The corneas of the mice were inoculated with HSV after scratching the corneal epithelium. Ten days after the inoculation, CTL were induced in vitro from
Induction of arginases I and II in cornea during herpes simplex virus infection.
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Induction of inducible nitric oxide synthase (iNOS) following corneal infection with herpes simplex virus type-1 (HSV-1) generates nitric oxide (NO), an important player in the defense against viral infection. Changes in arginine metabolism during infection are not limited to effects of iNOS but can
Quantification of stromal destruction in the inflamed cornea.
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An intrastromal injection of endotoxin lipopolysaccharide (LPS) in one eye of New Zealand albino rabbits induced a prominent keratitis characterized clinically and microscopically by edema and infiltration. Polymorphonuclear leukocytes (PMNs) constituted the primary invading leukocytic element.
Raman Spectroscopy Analysis of the Biochemical Characteristics of Experimental Keratomycosis.
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To investigate the biochemical characteristics in experimental keratomycosis by Raman spectroscopy analysis in vitro and in vivo.
Raman spectroscopy was used to analyze the biochemical characteristics of cultured mouse keratocytes stimulated by Fusarium solani suspension in vitro, and the infected
Missense mutation at the C-terminus of PAX6 negatively modulates homeodomain function.
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PAX6 is essential for ocular morphogenesis. Mutations in the PAX6 gene produce various phenotypes, including aniridia, Peters' anomaly, foveal hypoplasia, autosomal dominant keratitis and congenital cataracts. PAX6 functions as a transcription factor and has two DNA binding domains (a paired domain
Truncation mutations in the transactivation region of PAX6 result in dominant-negative mutants.
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PAX6 is a transcription factor with two DNA-binding domains (paired box and homeobox) and a proline-serine-threonine (PST)-rich transactivation domain. PAX6 regulates eye development in animals ranging from jellyfish to Drosophila to humans. Heterozygous mutations in the human PAX6 gene result in
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