The present invention involves the early diagnosis of cancerous or precancerous conditions in the gastrointestinal tract by detection of a backleak of signature proteins or carbohydrates in a biological sample obtained from the gastrointestinal tract.

Lankenau Institute for Medical Research

Early diagnosis of cancerous and precancerous conditions by leakage of signature peptides and carbohydrates into the bloodstream

An improved rectal mucus screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false negatives due to sampling error, immobilizes the mucus sample in a membrane filter, tests for marker carbohydrates which have vicinal galactose moieties and which are oxidized with galactose oxidase to vicinal aldehydic moieties which are visualized with Schiff's reagent and oxidizes those samples which test negative for the marker carbohydrates with periodic acid, renders the sample visualizable with Schiff's reagent, applies the galactose oxidase directly to the membrane filter, thereby speeding up color developed and employs the Schiff's Reagent which was refrigerated after preparation until the color faded to a straw shade before being treated with activated charcoal, which renders the solution storage stable ability for many months and enhances its color development ability.

Rectal mucus test and kit for detecting cancerous and precancerous conditions

An improved screening test method and kit for cancerous and precancerous conditions which is rapid, employs reagents which can be provided in kit form and which does not give false negatives due to sampling error, immobilizes a sample of a proteinaceous body fluid in a membrane filter, tests for marker carbohydrates therein which have vicinal galactose moieties and which are enzymatically oxidized with galactose oxidase to vicinal aldehydic moieties which then visualizes any thus-produced vicinal aldehydic moieties with Schiff's Reagent, then further oxidizes those samples which test negative for the marker carbohydrates with periodic acid, thereby rendering the sample visualizable with Schiff's Reagent if the sample contains glycoprotein, which applies the galactose oxidase directly to the membrane filter, thereby speeding up color developed, and employs Schiff's Reagent which was refrigerated after preparation until its color faded to a straw shade before being treated with activated charcoal, which treatment renders the solution storage-stable for many months and enhances its color development ability.

Screening test and kit for cancerous and precancerous conditions

A method for diagnostic or prognostic monitoring of premalignant or malignant conditions of human secretory epithelia, particularly colonic epithelia, by determining the extent of expression of a .beta.1-3N-acetylglucosaminyltransferase is described. Associated with the expression of a wide variety of carbohydrate antigens in adenocarcinomas is the induction of .beta.1-3N-acetylglucosaminyltransferase in epithelial cells. This enzyme is not found in normal, healthy adult colonic epithelial cells and thus indicates a novel and potentially sensitive method for screening the disease status of individuals.

precancerous conditions/carbohydrate

Early diagnosis of cancerous and precancerous conditions by leakage of signature peptides and carbohydrates into the bloodstream

Rectal mucus test and kit for detecting cancerous and precancerous conditions

Screening test and kit for cancerous and precancerous conditions

Diagnosis of cancerous or precancerous conditions in human secretory epithelia by enzyme activity of .beta.-1-3N-acetylglucosaminyltransferase

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