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Metabolic effects of citrate in liver during cold hypoxia studied by 1H NMR spectroscopy.
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We propose the use of 1H nuclear magnetic resonance (NMR) spectroscopy to investigate metabolite fluxes in the mammalian liver during cold hypoxia. Rat livers were flushed with one of four different preservation solutions and stored on ice in the same solution. The preservation solutions were:
Energy metabolism following prolonged hepatic cold preservation: benefits of interrupted hypoxia on the adenine nucleotide pool in rat liver.
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The ability of brief hypothermic reperfusion (HtR) to restore hepatic energy metabolism following periods of cold hypoxic preservation was studied in isolated rat livers after storage times of 5, 10, and 24 h. In addition, investigations were performed on the effects of HtR used to restore liver
Glycogen phosphorylase activity during the cold storage of liver: A limiting effect on glycolytic flux and energy production.
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This study examined the effects of dibutyryl-cyclic adenosine monophosphate (db-cAMP) and okadaic acid (a specific inhibitor of protein phosphatases 1 and 2A) as additives to a cold storage solution. The effects on levels of glycogen phosphorylase, the resultant effects on flux through the
[Addition of an osmotic agent to liver preservative solutions in a model of in vitro preservation of hepatocytes].
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To avoid hypoxic cell swelling during liver preservation followed by reduced perfusion in the reoxygenation period, osmotic substances such as mannitol, sucrose and raffinose, and the impermeant anion lactobionate are used in established liver preservation solutions. The various osmotic agents were
Hemoprotein formation in yeast. VI. Mutants with changed levels of catalase and of other heme enzymes under conditions of glucose repression.
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Three mutants with altered response of the hemoprotein level to glucose repression and anoxia were characterized biochemically. Two mutants show complete resistance of catalase activity to glucose repression and a lower degree of resistance of cytochrome c oxidase and of some other enzymes. Liquid
Isolation of xylose-assimilating yeasts and optimization of xylitol production by a new Meyerozyma guilliermondii strain.
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Production of xylitol from lignocellulosic biomass is of interest to modern biorefineries, because this biomass should be processed into a spectrum of chemicals (bio-based products) and not only energy. The isolation of new yeast strains capable of efficiently converting xylose into xylitol and
Sugar metabolism in the desiccation tolerant grass Oropetium thomaeum in response to environmental stresses.
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Oropetium thomaeum is a desiccation tolerant grass and acquisition of desiccation tolerance is correlated with changes in carbohydrate metabolism. Here we address the question whether the changes in carbohydrate metabolism are specific to the dehydration process or whether other environmental
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