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salmonella infections/nicotiana
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SseF, a type III effector protein from the mammalian pathogen Salmonella enterica, requires resistance-gene-mediated signalling to activate cell death in the model plant Nicotiana benthamiana.
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Type III effector proteins (T3Es) of many Gram-negative pathogenic bacteria manipulate highly conserved cellular processes, indicating conservation in virulence mechanisms during the infection of hosts of divergent evolutionary origin. In order to identify conserved effector functions, we used a
Production of human rotavirus and Salmonella antigens in plants and elicitation of fljB-specific humoral responses in mice.
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A Nicotiana benthamiana transient expression system was used to express single antigen and dimeric combinations of the human rotavirus (HRV) VP7 and a truncated VP4 (VP4Δ) proteins fused with Salmonella typhimurium's flagellin fljB subunit. Immunoblot analyses using rabbit antibodies generated
A Plant-Produced Bacteriophage Tailspike Protein for the Control of Salmonella.
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The receptor binding domain of the tailspike protein Gp9 from the P22 bacteriophage was recently shown to reduce Salmonella colonization in the chicken gut. In this study, we transiently expressed the receptor binding domain of the Gp9 tailspike protein in Nicotiana benthamiana, and targeted it to
Genetic toxicity of methyl methanethiosulfonate on Salmonella typhimurium, Saccharomyces cerevisiae and Nicotiana tabacum.
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The genetic toxicity of methyl methanethiosulfonate (MMT), a hydrolytic derivative of the insecticide methomyl, or lannate (Du Pont), was studied in Salmonella typhimurium, Saccharomyces cerevisiae and Nicotiana tabacum. At low concentrations, a lethal action was observed in all 3 models. Nuclear
Transgenic tobacco plants expressing a dimeric single-chain variable fragment (scfv) antibody against Salmonella enterica serotype Paratyphi B.
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Transgenic tobacco plants were produced that express an anti-Salmonella enterica single-chain variable fragment (scFv) antibody that binds to the lipopolysaccharide (LPS) of S. enterica Paratyphi B. The coding sequence of this scFv was optimized for expression in tobacco, synthesized and
Active suppression of early immune response in tobacco by the human pathogen Salmonella Typhimurium.
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The persistence of enteric pathogens on plants has been studied extensively, mainly due to the potential hazard of human pathogens such as Salmonella enterica being able to invade and survive in/on plants. Factors involved in the interactions between enteric bacteria and plants have been identified
Differential mutagenic response of Salmonella typhimurium to the plant-metabolized organophosphorus insecticides, phoxim and azinphos methyl.
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The plant cell/microbe coincubation assay was used to analyze organophosphorus insecticide activation. Salmonella typhimurium strains TA98 and TA100 were exposed to several concentrations of the pesticides phoxim and azinphos methyl with and without TX1 cell line of Nicotiana tabacum activation.
Expression of a bacterial aroA mutant, aroA-M1, encoding 5-enolpyruvylshikimate-3-phosphate synthase for the production of glyphosate-resistant tobacco plants.
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Glyphosate is a non-selective broad-spectrum herbicide that inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme in the aromatic amino acid biosynthetic pathway in microorganisms and plants. We have previously reported a strategy for engineering glyphosate-resistant class I
Experimental studies on mutagenic and carcinogenic effects of tobacco chewing.
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The alcoholic extract of the chewing (Pandharpuri) variety of tobacco (Nicotiana tabacum) was subjected to mutagenicity and tumorigenicity studies. The extract was found to be mutagenic in strain TA 98 of Salmonella typhimurium in the presence of S 9 mixture. It also induced 8 AZG-resistant mutation
[Construction and expression of vector with aroA-in gene and its transformation in tobacco].
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Inteins are intervening protein sequences that undergo self-excision from precursor protein with concomitant joining of flanking sequences. Here, we demonstrated intein cis-splicing in Nicotiana tabacum nuclear genomes by using artificial cis Ssp DnaB and Rma DnaB intein. We want to test whether
A plastidial pantoate transporter with a potential role in pantothenate synthesis.
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The pantothenate (vitamin B5) synthesis pathway in plants is not fully defined because the subcellular site of its ketopantoate → pantoate reduction step is unclear. However, the pathway is known to be split between cytosol, mitochondria, and potentially plastids, and inferred to involve
The gene expression and enzyme activity of plant 3-deoxy-D-manno-2-octulosonic acid-8-phosphate synthase are preferentially associated with cell division in a cell cycle-dependent manner.
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3-deoxy-D-manno-2-octulosonic acid-8-phosphate (Kdo-8-P) synthase catalyzes the condensation of phosphoenolpyruvate with D-arabinose-5-phosphate to yield Kdo-8-P. Kdo-8-P is the phosphorylated precursor of Kdo, a rare sugar only found in the rhamnogalacturonan II pectic fraction of the primary cell
Flagellin produced in plants is a potent adjuvant for oral immunization.
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The aim of this study was to produce adjuvant with high biosafety, efficacy and low cost. Towards this goal, the plant Nicotiana benthamiana transient expression system was successfully used to express Salmonella typhimurium's flagellin (FljB). The yield of the expressed FljB was 280 mg per kg of
Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin.
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The development of recombinant influenza vaccines with broad spectrum protection is an important task. The combination of conservative viral antigens, such as M2e, the extracellular domain of the transmembrane protein M2, and conserved regions of the second subunit of hemagglutinin (HA), provides an
Fruit Rot of Tinda Caused by Pseudomonas aeruginosa-A New Report from India.
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Fruit rot disease (FRD), an emerging problem of tinda (Praecitrullus fistulosus) in India. FRD epidemics begin during rainy and warm weather and often spoil marketable produce. Symptoms appear as numerous, pale brown-to-dark brown, deeply penetrating circular soft rot lesions on fleshy fruit
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