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Página 1 desde 45 resultados
Nucleotide Metabolism in Salt-Stressed Zea mays L. Root Tips: I. Adenine and Uridine Nucleotides.
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Corn plants (Zea mays L. cv Pioneer 3906) were grown in a glass house on control and saline nutrient solutions, in winter and summer. There were two saline treatments, both with osmotic potential = -0.4 megapascal but with different Ca(2+)/Na(+) ratios: 0.03 and 0.73. Root tips and shoot meristems
Non-allosteric regulation of the uridine kinase from seeds of Zea mays.
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Uridine kinase (ATP: uridine 5'-phosphotransferase, EC 2.7.1.48) has been partially purified from ungerminated hybrid corn seed. It is associated with a soluble high molecular weight fraction from which it apparently cannot be dissociated without loss of activity. The stability of the enzyme is
Properties of uridine and thymidine phosphorylating enzymes of Zea mays.
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Cytological study on water stress during germination of Zea mays.
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Kernels of Zea mays were subjected to dehydration treatment at various times during germination. Embryos from kernels dehydrated during the first 36 h of germination are resistant to dehydration and subsequently germinate earlier than controls. Dehydration of kernels germinated during 72h leads to
Measurement of Metabolites Associated with Nonaqueously Isolated Starch Granules from Immature Zea mays L. Endosperm.
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Starch granules with associated metabolites were isolated from immature Zea mays L. endosperm by a nonaqueous procedure using glycerol and 3-chloro-1,2-propanediol. The soluble extract of the granule preparation contained varying amounts of neutral sugars, inorganic phosphate, hexose and triose
RNA synthesis and changes in template--DNA activity during the growth and differentiation of parenchyma cells of the primary cortex of roots of Zea mays and Tulipa kaufmanniana.
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In this study, changes in DNA transcription activity are presented during parenchymal cell differentiation of the primary cortex of roots of a species with elevated DNA content in which endomitotic polyploidization does not occur (Tulipa kaufmanniana), as well as a species with low content levels in
Characterization of ribonucleic acid synthesis by nuclei isolated from Zea mays.
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Nuclei were isolated from the shoots of Zea mays and assayed for endogenous RNA polymerase activity in vitro. Maximum incorporation from radioactive precursors (70 pmol [(3)H]uridine 5' monophosphate/100 μg DNA) was reached after incubation for 1 h at 25°C. The RNA product, analysed by
Enzymic synthesis of indole-3-acetyl-1-O-beta-d-glucose. I. Partial purification and characterization of the enzyme from Zea mays.
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The first enzyme-catalyzed reaction leading from indole-3-acetic acid (IAA) to the myo-inositol esters of IAA is the synthesis of indole-3-acetyl-1-O-beta-D-glucose from uridine-5'-diphosphoglucose (UDPG) and IAA. The reaction is catalyzed by the enzyme, UDPG-indol-3-ylacetyl glucosyl transferase
iaglu, a gene from Zea mays involved in conjugation of growth hormone indole-3-acetic acid.
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Plants contain most of the growth hormone indole-3-acetic acid (IAA) in conjugated forms believed to be inactive in promoting growth. The iaglu gene, which controls the first step in the biosynthesis of the IAA conjugates of Zea mays, encodes (uridine
Small kernel 1 encodes a pentatricopeptide repeat protein required for mitochondrial nad7 transcript editing and seed development in maize (Zea mays) and rice (Oryza sativa).
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RNA editing modifies cytidines (C) to uridines (U) at specific sites in the transcripts of mitochondria and plastids, altering the amino acid specified by the DNA sequence. Here we report the identification of a critical editing factor of mitochondrial nad7 transcript via molecular characterization
[RNA synthesis and translocation in root cells of Zea mays during early stages of germination].
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RNA synthesis in root of germinating corn during the period extending from the 4th to the 8th hr after soaking is localized exclusively in the chromatin. This unusual situation allowed us to follow easily by section autoradiography the fate of the chromatin-synthesized RNA. The embryo was
Root cell ultrastructure of Zea mays embryo during early stages of germination.
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The ultrastructure of root cells of the germinating corn embryo has been studied during the first 72 hours of soaking. The most spectacular ultrastructural modifications occur in the nucleus. In the dry seed, the chromatin is heavily condensed and complete dispersion occurs during the first 8 hr of
Growth Inhibition and Metabolite Pool Levels in Plant Tissues Fed d-Glucosamine and d-Galactose.
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The growth of corn (Zea mays) roots and barley (Hordeum vulgare) coleoptiles is sensitive to the presence of external d-glucosamine and d-galactose. In order to investigate this effect, tissues were fed the radioactive monosaccharides at concentrations that ranged from those that were strongly
Effect of light on ribonucleic Acid metabolism in greening maize leaves.
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The effect of illumination on the incorporation of labeled precursors into RNA of dark-grown maize (Zea mays) leaves was studied using either (32)P-phosphate or double labeling with (14)C- and (3)H-uridine. In the dark, label was preferentially incorporated into etioplast ribosomal RNAs.
Newly Synthesized mRNA Is Translated during the Initial Imbibition Phase of Germinating Maize Embryo.
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RNA synthesis is activated in the cells of the plant embryo very soon after the start of seed imbibition. We previously reported that mainly heterogeneous nuclear RNA is synthesized in the radicle of Zea mays embryo during the first hours of germination. The present study was undertaken in order to
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