First Report of a Geminivirus Associated with Leaf Curl in Baja California Peninsula Tomato Fields.

Since November 2001, geminivirus-like symptoms (stunting, reduced leaf size, and leaf curling "chino") have been observed in tomato (Lycopersicon esculentum Mill.) plantings in Baja California Sur, Mexico. Samples of symptomatic plants were collected from commercial fields and analyzed by traditional and molecular methods for the presence of geminiviruses. Inocula prepared from infected plants were experimentally transmitted to tomato seedlings and Datura stramonium by mechanical inoculation and whitefly transmission. Leaf curling and interveinal chlorosis symptoms similar to those found in the field were observed in inoculated tomato and D. stramonium. DNA from infected plants was extracted and analyzed by polymerase chain reaction (PCR) and electrophoresis using degenerate primers PALIv1978/PARIc494 (1). PCR fragments of the expected size (1.1 kb) for the common region (CR) were obtained from 28 of 64 plants, cloned and sequenced (GenBank Accession No. AY336088). Comparisons of CR sequences with the NCBI database by using BLAST and MegAlign (DNASTAR, London) indicated that the Baja Californian isolates were New World bipartite begomoviruses sharing the highest nucleotide sequence identity (93%) with a partially characterized geminivirus (Tomato severe leaf curl virus (ToSLCV); GenBank Accession No. AF130415) from Guatemala. References: (1) M. R. Rojas et al. Plant Dis. 77:340, 1993.