Hepatoprotective activity of glucosyloxybenzyl succinate derivatives from the pseudobulbs of Pleione bulbocodioides.

Ten previously undescribed glucosyloxybenzyl succinate derivatives, pleionosides A-J, and fifteen known compounds were isolated from the pseudobulbs of Pleione bulbocodioides (Franch.) Rolfe. Their structures were elucidated by a combination of MS and NMR data, physical and chemical methods and a comparison with known compounds. Furthermore, three compounds exhibited potent hepatoprotective activity against N-acetyl-p-aminophenol (APAP)-induced HepG2 cell damage in in vitro assays, with cell survival rates of 31.89%, 31.52% and 31.97% at 10 μM. Four compounds exhibited moderate antioxidant activity with increasing viability at 10 μM of 36.1%, 45.0%, 25.5% and 20.7%.