[Study on Panax notoginseng mobilizing marrow stem cells efferens efficiency of acute myocardial infarction in rats].

OBJECTIVE

To study the mobilization efficiency effect on bone marrow stem cells by Panax notoginseng on acute myocardial infarction in experimental rats.

METHODS

One hundred and thirty wistar clean rats with average age 16 weeks weighing (200 +/- 10) g, half female, ligated anterior descending coronary artery proximal,were established animal model of acute myocardial infarction. They were randomly divided into: model group, Chinese medicine mobilization group, western medicine mobilization group, integrated Chinese and western medicine mobilization group and sham group. Except sham group rats, other groups after the survival of 24 h by 1, 7, 14 d, and other points in time were divided into three subgroups. They were injected drugs immediately 24 h after the surgery. Injected G-CSF 50 ug x kg(-1) x d(-1) in western medicine group subcutaneously, NS 50 g x kg(-1) x d(-1) in sham group and model group. In Chinese medicine mobilization group intraperitoneally injected notoginsenoside (150 mg x kg(-1)). Chinese and western medicines mobilization group were subcutaneously injected G-CSF 5 microg x kg(-1) x d(-1) and intraperitoneally injected notoginsenoside (150 mg x kg(-1)), with G-CSF once a day, 7 days totally and G-CSF once a day until each sub-group of animals were killed. Severn rats were remained for experiments in every subgroup. Flow cytometry were used to detect the percentage of peripheral blood mononuclear cells and white blood, C-kit receptor expression of peripheral blood mononuclear cells.

RESULTS

WBC, MNC% of model group 1, 7, 14 d were significantly larger than those of sham group 1, 7, 14 d (P < 0.05), which indicates that MI animals can mobilize themselves. WBC and MNC% of Chinese medicine group and western medicine group were lower than those of Chinese and western medicine group in 1, 7, 14 d (P < 0.05). C-kit receptor expression in PBMC of Chinese and western medicine group and western medicine group were higher than those of model group. Western medicine is better than Chinese medicine in mobilization.

CONCLUSIONS

: Panax notoginseng may cooperation G-CSF with mobilizing stem cell, raise the efferens efficiency of bone marrow stem cells, increase the number of peripheral blood stem cells.