The role of alpha 1-proteinase inhibitor in semisoluble glucan induced resistance to hemorrhage.

The present study was undertaken to evaluate the mechanism of protective effect of semisoluble beta-1,3-carboxymethylglucan (CMG) during acute massive hemorrhage. CBA mice were injected i.v. with glucan (25 mg/kg) 24 h prior to hemorrhage (50% of blood circulating volume). Survival data indicated that glucan increased survival compared with control. However, alpha 1-proteinase inhibitor activity in serum have been even decreased 24 h after glucan administration. Moreover enhancement of active oxygen form production and leakage of cytosol and lysosomal enzymes after CMG application were noted.