glutamate dehydrogenase/riis

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Overexpression of an NADP(H)-dependent glutamate dehydrogenase gene, TrGDH, from Trichurus improves nitrogen assimilation, growth status and grain weight per plant in rice.

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As glutamate dehydrogenases (GDHs) of microorganisms usually have higher affinity for NH₄⁺ than do those of higher plants, it is expected that ectopic expression of these GDHs can improve nitrogen assimilation in higher plants. Here, a novel NADP(H)-GDH gene (TrGDH) was

Over-expression of a glutamate dehydrogenase gene, MgGDH, from Magnaporthe grisea confers tolerance to dehydration stress in transgenic rice.

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CONCLUSIONS Heterologous expression of a fungal NADP(H)-GDH gene ( MgGDH ) from Magnaporthe grisea can improve dehydration stress tolerance in rice by preventing toxic accumulation of ammonium. Glutamate dehydrogenase (GDH; EC 1.4.1.2 and EC 1.4.1.4) may act as a stress-responsive enzyme in

Assimilation of exogenous cyanide cross talk in Oryza sativa L. to the key nodes in nitrogen metabolism

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Exogenous cyanide (CN^-) effects on nitrogen (N) uptake, transport, and assimilation in rice seedlings were investigated at the biochemical and molecular levels. Seedlings were treated with either a 2-d or 4-d supply of potassium cyanide (KCN) in the nutrient solution containing nitrate

Nitrogen metabolism correlates with the acclimation of photosynthesis to short-term water stress in rice (Oryza sativa L.).

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Nitrogen metabolism is as sensitive to water stress as photosynthesis, but its role in plant under soil drying is not well understood. We hypothesized that the alterations in N metabolism could be related to the acclimation of photosynthesis to water stress. The features of photosynthesis and N

Effects of nitric oxide on nitrogen metabolism and the salt resistance of rice (Oryza sativa L.) seedlings with different salt tolerances

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Salt stress inhibits rice productivity seriously. Nitric oxide (NO) is an endogenous signaling molecule in plants that can improve the resistance of rice to abiotic stresses. Previous studies also showed that nitrogen metabolism is essential for rice stress-tolerance. However, the physiological and

Molecular cloning, characterization and function analysis of a GDH gene from Sclerotinia sclerotiorum in rice.

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The full-length cDNA encoding a glutamate dehydrogenase (GDH) which catalyzes the reaction of reductive amination of α-oxoglutarate (α-OG) to glutamate (the anabolic activity) and the reverse reaction of oxidative deamination of glutamate (the catabolic activity) was isolated from Sclerotinia

Refining the definition of plant mitochondrial presequences through analysis of sorting signals, N-terminal modifications, and cleavage motifs.

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Mitochondrial protein import is a complex multistep process from synthesis of proteins in the cytosol, recognition by receptors on the organelle surface, to translocation across one or both mitochondrial membranes and assembly after removal of the targeting signal, referred to as a presequence. In

Intracellular distribution of enzymes associated with nitrogen assimilation in roots.

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The cellular distribution of enzymes involved in nitrogen assimilation: nitrate reductase (EC 1.6.6.2), nitrite reductase (EC 1.6.6.4), glutamine synthetase (EC 6.3.1.2), glutamate synthase (EC 2.6.1.53), and glutamate dehydrogenase (EC 1.4.1.3) has been studied in the roots of five plants: maize

Aspects of nitrogen metabolism in the rice seedling.

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The effects of nitrogen source NO(3) (-) or NH(4) (+) on nitrogen metabolism during the first 2 weeks of germination of the rice seedling (Oryza sativa L., var. IR22) grown in nutrient solution containing 40 mug/ml N were studied. Total, soluble protein, and free amino N levels were higher in the

Protein metabolism in leaves and developing grains of rices differing in grain protein content.

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Four semi-dwarf rices (Oryza sativa L.) differing in percentage of grain protein, grown in a flooded field receiving basal N fertilization, had a peak activity of root glutamate dehydrogenase 4 weeks after transplanting. A lower peak occurred during panicle formation 10 weeks after transplanting.

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