[Effect of a new gasotransmitter, hydrogen sulfide, on collagen remodeling of pulmonary artery under hypoxia].
کلید واژه ها
خلاصه
OBJECTIVE
To study the modulatory effect of hydrogen sulfide (H(2)S) on the accumulation of collagen type I and type III in the wall of pulmonary small artery during hypoxic pulmonary vascular remodeling.
METHODS
Nineteen male Wistar rats were randomly divided into a control group (n = 6), a hypoxic group (n = 7) and a hypoxia + NaHS group (n = 6). Hypoxic challenge was performed everyday for 21 days. NaHS solution was injected peritoneally everyday before hypoxia challenge for rats in the hypoxia + NaHS group. After 21 days of hypoxia, the mean pulmonary artery pressure was measured by pulmonary artery catheterization. The weight ratio of right ventricle to left ventricle + septum [RV/(LV + SP)] was also measured. The plasma level of H(2)S was determined by methylene blue spectrophotometric method. The expression of collagen type I and type III in pulmonary small arteries were detected by immunohistochemistry. The expression of procollagen type I and type III mRNA in pulmonary small arteries were detected by in situ hybridization.
RESULTS
(1) Compared with the control group, the mPAP increased by 46% (P < 0.01), the weight ratio of RV/(LV + SP) increased by 41% and the plasma level of H(2)S decreased by 36% for rats in the hypoxia group (P < 0.01). Compared with the hypoxia group, the mPAP decreased by 31% (P < 0.01), the weight ratio of RV/(LV + SP) decreased by 24% and the plasma level of H(2)S increased by 65% (P < 0.01) for rats in the hypoxia + NaHS group. (2) Expression of collagen type I in small and median pulmonary arteries of the three groups: compared with rats in the control group, collagen type I expression increased by 81% and 62% respectively for rats in the hypoxia group (P < 0.01); compared with rats in the hypoxia group, the expression decreased by 32% and 18% respectively for rats in the hypoxia + NaHS group (P < 0.01). (3) Expression of procollagen type I mRNA in small and mid pulmonary arteries of the three groups: compared with rats in the control group, the expression increased by 49% and 68% respectively (P < 0.01) for rats in the hypoxia group; compared with rats in the hypoxia group, the expression decreased by 31% and 33% respectively for rats in the hypoxia + NaHS group (P < 0.01). (4) Expression of collagen type III in small pulmonary arteries of the three groups: compared with rats in the control group, the expression increased by 84% for rats in the hypoxia group (P < 0.01); compared with rats in the hypoxia group, the expression decreased by 37% for rats in the hypoxia + NaHS group (P < 0.01). Compared with rats in the control group, the expression of collagen type III in median pulmonary arteries increased by 38% in the hypoxia group (P < 0.01), while there was no significant difference between the hypoxia group and the hypoxia + NaHS group. (5) Expression of procollagen type III mRNA in small and median pulmonary arteries of the three groups: compared with rats in the control group, the expression increased by 53% and 17% respectively (P < 0.01) for rats in the hypoxia group; compared with rats in the hypoxia group, the expression decreased by 45% and 33% respectively for rats in the hypoxia + NaHS group (P < 0.01).
CONCLUSIONS
In the process of hypoxic pulmonary vascular collagen remodeling in rats, H(2)S could inhibit the abnormal accumulation of collagen type I and type III in the wall of pulmonary small arteries. This effect may be one of the mechanisms by which H(2)S ameliorates hypoxic pulmonary vascular remodeling.
